Comparison of the peptidome and insecticidal activity of venom from a taxonomically diverse group of theraphosid spiders

Gentz, M. C., Jones, A, Clement, H and King, G. F. (2009) Comparison of the peptidome and insecticidal activity of venom from a taxonomically diverse group of theraphosid spiders. Toxicon, 53 5: 496-502. doi:10.1016/j.toxicon.2009.01.025


Author Gentz, M. C.
Jones, A
Clement, H
King, G. F.
Title Comparison of the peptidome and insecticidal activity of venom from a taxonomically diverse group of theraphosid spiders
Journal name Toxicon   Check publisher's open access policy
ISSN 0041-0101
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.toxicon.2009.01.025
Open Access Status Not yet assessed
Volume 53
Issue 5
Start page 496
End page 502
Total pages 7
Editor Alan L. Harvey
Place of publication Amsterdam
Publisher Elsevier
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
0703 Crop and Pasture Production
070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Abstract We screened a panel of theraphosid venoms in two orders of insect in order to determine whether these bioassays would help in the selection of candidate venoms for future discovery of insecticidal toxins. Venoms from six different theraphosid genera were compared with venom from the Australian funnel-web spider Hadronyche infensa (Hexathelidae). The tarantulas included were Coremiocnemis tropix, Selenocosmia crassipes, and Selenotholus foelschei from Australia and Brachypelma albiceps and Brachypelma hamorii from Mexico. The insects assayed, Tenebrio molitor (Coleoptera: Tenebrionidae) and Acheta domesticus (Orthoptera: Gryllidae), were selected because of their relevance as model holometabolous and hemimetabolous insects, respectively, as well as their taxonomic relationship to economically important pest insects. Despite significant differences in their peptide/protein profiles as determined using SDS-PAGE, HPLC, and mass spectrometry, all of the theraphosid venoms exhibited remarkably similar LD50 values of 46–126 μg/g for crickets and 0.5–4.0 μg/g for mealworms. Notably, mealworms were on average 50-fold more susceptible than crickets to each of the crude theraphosid venoms and consequently they provide an excellent bioassay system when venom supply is limited. This study indicates that even closely related spiders have evolved quite different toxin repertoires that nevertheless have comparable efficiency with respect to killing their primary prey, namely insects.
Keyword Spider
Venom
Insecticidal toxin
Tarantula
Australian funnel-web spider
venom peptidome
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 18:19:40 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience