Expression of PAL and HSPs in fresh-cut banana fruit

Chen, J.Y., He, L.H., Jiang, Y.M., Kuang, J.F., Lu, C.B., Joyce, D.C., Macnish, A., He, Y.X. and Lu, W.J. (2009) Expression of PAL and HSPs in fresh-cut banana fruit. Environmental And Experimental Botany, 66 1: 31-37. doi:10.1016/j.envexpbot.2008.12.019

Author Chen, J.Y.
He, L.H.
Jiang, Y.M.
Kuang, J.F.
Lu, C.B.
Joyce, D.C.
Macnish, A.
He, Y.X.
Lu, W.J.
Title Expression of PAL and HSPs in fresh-cut banana fruit
Journal name Environmental And Experimental Botany   Check publisher's open access policy
ISSN 0098-8472
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.envexpbot.2008.12.019
Open Access Status Not yet assessed
Volume 66
Issue 1
Start page 31
End page 37
Total pages 7
Editor J C Kader
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
820214 Tropical Fruit
070605 Post Harvest Horticultural Technologies (incl. Transportation and Storage)
Abstract Wounding of plants is a widespread stress in nature. Physical damage frequently occurs during harvesting, handling and marketing of fresh produce. Phenylalanine ammonia-lyase (PAL) is a key enzyme involved in plant wound related phenylpropanoid metabolism while heat shock proteins (HSPs) are involved in abiotic, including wounding, and biotic stress responses in plants. However, little is known about postharvest expression at gene and protein levels of PAL and HSPs in wounding (fresh-cut) fruits. In the present study, expression patterns of PAL and HSPs in fresh-cut and intact banana fruit pulp were characterized by Northern and Western blot analyses. Cuting enhanced Ma-PAL2 mRNA accumulation, new PAL protein synthesis, and PAL activity; thereby resulting in accumulation of total phenolics. HSP70 protein and Ma-HSP70-2 transcript were constitutively expressed and were not affected by cutting. However, Ma-HSP70-1 transcript levels increased in fresh-cut pulp tissue at 2–18 h after cutting. Ma-sHSP-1-CI, Ma-sHSP-3-CII, Ma-HSP90, and Ma-HSP101 transcripts also accumulated in fresh-cut tissues at 2–18 h after cuting, but Ma-sHSP-2-CI did not accumulate. Accumulation of sHSP (HSP17.6), HSP90 and HSP101 proteins exhibited the same pattern of expression as their mRNA. There was no evidence of a possible suppressive effect of HSPs synthesis on PAL protein accumulation. Thus, PAL, sHSP, HSP90 and HSP101 were regulated by cutting both at translational and transcriptional levels while HSP70 (Ma-HSP70-1) was regulated by cutting only at transcription level.
Keyword Banana
Phenylalanine ammonia-lyase
Heat shock proteins
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 30571299
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 18:18:47 EST by Mr Andrew Martlew on behalf of School of Land, Crop and Food Sciences