Generation of cubic membranes by controlled homotypic interaction of membrane proteins in the endoplasmic reticulum

Lingwood, Daniel, Schuck, Sebastian, Ferguson, Charles and Gerl, Mathias J. (2009) Generation of cubic membranes by controlled homotypic interaction of membrane proteins in the endoplasmic reticulum. Journal of Biological Chemistry, 284 18: 12041-12048. doi:10.1074/jbc.M900220200

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Author Lingwood, Daniel
Schuck, Sebastian
Ferguson, Charles
Gerl, Mathias J.
Title Generation of cubic membranes by controlled homotypic interaction of membrane proteins in the endoplasmic reticulum
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 2009-05-01
Sub-type Article (original research)
DOI 10.1074/jbc.M900220200
Open Access Status File (Publisher version)
Volume 284
Issue 18
Start page 12041
End page 12048
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Cell membranes predominantly consist of lamellar lipid bilayers. When studied in vitro, however, many membrane lipids can exhibit non-lamellar morphologies, often with cubic symmetries. An open issue is how lipid polymorphisms influence organelle and cell shape. Here, we used controlled dimerization of artificial membrane proteins in mammalian tissue culture cells to induce an expansion of the endoplasmic reticulum (ER) with cubic symmetry. Although this observation emphasizes ER architectural plasticity, we found that the changed ER membrane became sequestered into large autophagic vacuoles, positive for the autophagy protein LC3. Autophagy may be targeting irregular membrane shapes and/or aggregated protein. We suggest that membrane morphology can be controlled in cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 18:16:23 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience