Neonatal blood cultures: Effect of delayed entry into the blood culture machine and bacterial concentration on the time to positive growth in a simulated model

Jardine, Luke Anthony, Sturgess, Barabra Ruth, Inglis, Garry Donald Trevor and Davies, Mark William (2009) Neonatal blood cultures: Effect of delayed entry into the blood culture machine and bacterial concentration on the time to positive growth in a simulated model. Journal of Paediatrics and Child Health, 45 4: 210-214. doi:10.1111/j.1440-1754.2008.01455.x


Author Jardine, Luke Anthony
Sturgess, Barabra Ruth
Inglis, Garry Donald Trevor
Davies, Mark William
Title Neonatal blood cultures: Effect of delayed entry into the blood culture machine and bacterial concentration on the time to positive growth in a simulated model
Journal name Journal of Paediatrics and Child Health   Check publisher's open access policy
ISSN 1034-4810
1440-1754
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1440-1754.2008.01455.x
Open Access Status
Volume 45
Issue 4
Start page 210
End page 214
Total pages 5
Editor David Isaacs
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject C1
920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified
110801 Medical Bacteriology
Abstract Aims: To determine if: time from blood culture inoculation to positive growth (total time to positive) and time from blood culture machine entry to positive growth (machine time to positive) is altered by delayed entry into the automated blood culture machine, and if the total time to positive differs by the concentration of organisms inoculated into blood culture bottles. Methods: Staphylococcus epidermidis, Escherichia coli and group B beta-haemolytic streptococci were chosen as clinically significant representative organisms. Two concentrations (≥10 colony-forming units per millilitre and <1 colony-forming units per millilitre) were inoculated into PEDS BacT/Alert blood culture bottles and randomly allocated to one of three delayed automated blood culture machine entry times (30 min/8.5 h/15.5 h). Results: For all organisms at all concentrations, except the Staphylococcus epidermidis, the machine time to positive was significantly decreased by delayed entry. For all organisms at all concentrations, the mean total time to positive significantly increased with increasing delayed entry into the blood culture machine. Higher concentrations of group B beta-haemolytic streptococci and Escherichia coli grew significantly faster than lower concentrations. Conclusion: Bacterial growth in inoculated bottles, stored at room temperature, continues although at a slower rate than in those blood culture bottles immediately entered into the machine. If a blood culture specimen has been stored at room temperature for greater than 15.5 h, the currently allowed safety margin of 36 h (before declaring a result negative) may be insufficient.
Keyword bacteremia
incubation time
bacteriological techniques
blood cultures
infant, newborn
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Thu, 03 Sep 2009, 18:16:08 EST by Mr Andrew Martlew on behalf of Paediatrics & Child Health - RBWH