Characterization and binding affinities of SmLANP: A new Schistosoma mansoni member of the ANP32 family of regulatory proteins

Pinheiro, C. S., Staub, O., Mulvenna, J., Loukas, A., Jones, M. K. and Rabelo, E. M. (2009) Characterization and binding affinities of SmLANP: A new Schistosoma mansoni member of the ANP32 family of regulatory proteins. Molecular and Biochemical Parasitology, 165 2: 95-102. doi:10.1016/j.molbiopara.2009.01.009


Author Pinheiro, C. S.
Staub, O.
Mulvenna, J.
Loukas, A.
Jones, M. K.
Rabelo, E. M.
Title Characterization and binding affinities of SmLANP: A new Schistosoma mansoni member of the ANP32 family of regulatory proteins
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.molbiopara.2009.01.009
Open Access Status
Volume 165
Issue 2
Start page 95
End page 102
Total pages 8
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Language eng
Subject 1312 Molecular Biology
2405 Parasitology
Abstract Members of the leucine-rich repeat protein family are involved in diverse functions including protein phosphatase 2-inhibition, cell cycle regulation, gene regulation and signalling pathways. A novel Schistosoma mansoni gene, called SmLANP, presenting homology to various genes coding for proteins that belong to the super family of leucine-rich repeat proteins, was characterized here. SmLANP was 1184 bp in length as determined from cDNA and genomic sequences and encoded a 296 amino acid open reading frame that spanning from 6 to 894 bp. The predicted amino acid sequence had a calculated molecular weight of 32 kDa. Analysis of the predicted sequence indicated the presence of 3 leucine-rich domains (LRR) located in the N-terminal region and an aspartic acid rich region in the C-terminal end. SmLANP transcript is expressed in all stages of the S. mansoni life cycle analyzed, exhibiting the highest expression level in males. The SmLANP protein was expressed in a GST expression system and antibodies raised in mice against the recombinant protein. By immunolocalization assay, using adult worms, it was shown that the protein is mainly present in the cell nucleus through the whole body and strongly expressed along the tegument cell body nuclei of adult worms. As members of this family are usually involved in protein-protein interaction, a yeast two hybrid assay was conducted to identify putative binding partners for SmLANP. Thirty-six possible partners were identified, and a protein ATP synthase subunit alpha was confirmed by pull down assays, as a binding partner of the SmLANP protein. (C) 2009 Elsevier B.V. All rights reserved.
Keyword Schistosoma mansoni
Amyloid precursor protein
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID CBB-2205/96
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Thu, 03 Sep 2009, 18:15:33 EST by Mr Andrew Martlew on behalf of School of Medicine