A Study on Nucleation for Protein Crystallization in Mixed Vessels

Tait, S., White, E. T. and Litster, J. D. (2009) A Study on Nucleation for Protein Crystallization in Mixed Vessels. CRYSTAL GROWTH & DESIGN, 9 5: 2198-2206. doi:10.1021/cg8009145

Author Tait, S.
White, E. T.
Litster, J. D.
Title A Study on Nucleation for Protein Crystallization in Mixed Vessels
Journal name CRYSTAL GROWTH & DESIGN   Check publisher's open access policy
ISSN 1528-7483
Publication date 2009-03-16
Year available 2009
Sub-type Article (original research)
DOI 10.1021/cg8009145
Open Access Status Not yet assessed
Volume 9
Issue 5
Start page 2198
End page 2206
Total pages 9
Editor Rogers, R. D.
Place of publication United States
Publisher American Chemical Society
Language eng
Subject C1
090406 Powder and Particle Technology
970109 Expanding Knowledge in Engineering
Abstract This study investigated mechanisms of secondary nucleation for the crystallization of the protein hen egg white lysozyme. A parametric study of the secondary nucleation of lysozyme in a stirred tank crystallizer was performed, testing the influence of stirrer speed, the size and volume of added seed crystals, solution temperature, supersaturation, and sodium chloride concentration on measured nucleation rates. The measured rates were highly variable, which made assessment of the influence of experimental parameters difficult. Only stirrer speed exhibited significant influence over experimental variability (10-fold increase in nucleation rates with a 50% increase in stirring speed). These results were interpreted by comparison with parameter influence that is commonly observed with secondary nucleation of nonproteins. A novel tubular crystallizer was used to assess the influence on secondary nucleation of fluid shear forces acting on lysozyme crystals. It was observed that fluid shear forces acting on mounted lysozyme crystals do not notably influence secondary nucleation. Overall, the results suggest that attrition is an important source of secondary nuclei in lysozyme crystallization and that secondary nucleation caused solely by fluid shear forces is not.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemical Engineering Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 18:10:18 EST by Mr Andrew Martlew on behalf of School of Chemical Engineering