Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides

Morgan, Jess A.T., Corley, Sean W., Jackson, Louise A., Lew-Tabor, Ala E., Moolhuijzen, Paula M. and Jonsson, Nicholas N. (2009) Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides. International Journal For Parasitology, 39 7: 775-779. doi:10.1016/j.ijpara.2008.12.006


Author Morgan, Jess A.T.
Corley, Sean W.
Jackson, Louise A.
Lew-Tabor, Ala E.
Moolhuijzen, Paula M.
Jonsson, Nicholas N.
Title Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides
Journal name International Journal For Parasitology   Check publisher's open access policy
ISSN 0020-7519
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2008.12.006
Open Access Status Not yet assessed
Volume 39
Issue 7
Start page 775
End page 779
Total pages 5
Place of publication United States
Publisher Elsevier Science
Language eng
Abstract Resistance against synthetic pyrethroid (SP) products for the control of cattle ticks in Australia was detected in the field in 1984, within a very short time of commercial introduction. We have identified a mutation in the domain II S4-5 linker of the para-sodium channel that is associated with resistance to SPs in the cattle tick Rhipicephalus (Boophilus) microplus from Australia. The cytosine to adenine mutation at position 190 in the R. microplus sequence AF134216, results in an amino acid substitution from leucine in the susceptible strain to isoleucine in the resistant strain. A similar mutation has been shown to confer SP resistance in the whitefly, Bemisia tabaci, but has not been described previously in ticks. A diagnostic quantitative PCR assay has been developed using allele-specific Taqman (R) minor groove-binding (MGB) probes. Using the assay to screen field and laboratory populations of ticks showed that homezygote allelic frequencies correlated highly with the survival percentage at the discriminating concentration of cypermethrin. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Keyword Rhipicephalus microplus
Probe assay
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Thu, 03 Sep 2009, 18:09:52 EST by Mr Andrew Martlew on behalf of School of Veterinary Science