DNA elution from buccal cells stored on Whatman FTA classic cards using a modified methanol fixation method

Johanson, H. C., Hyland, V, Wicking, C. A. and Sturm, R. A. (2009) DNA elution from buccal cells stored on Whatman FTA classic cards using a modified methanol fixation method. Bio Techniques, 46 4: 309-311. doi:10.2144/000113077


Author Johanson, H. C.
Hyland, V
Wicking, C. A.
Sturm, R. A.
Title DNA elution from buccal cells stored on Whatman FTA classic cards using a modified methanol fixation method
Journal name Bio Techniques   Check publisher's open access policy
ISSN 0736-6205
1940-9818
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.2144/000113077
Open Access Status Not Open Access
Volume 46
Issue 4
Start page 309
End page 311
Total pages 3
Editor Douglas McCormick
Place of publication New York , U.S.A
Publisher Informa Healthcare
Language eng
Subject 1004 Medical Biotechnology
C1
920117 Skin and Related Disorders
060199 Biochemistry and Cell Biology not elsewhere classified
Abstract We describe here a method for DNA elution from buccal cells and whole blood both collected onto Whatman FTA technology, using methanol fixation followed by an elution PCR program. Extracted DNA is comparable in quality to published Whatman FTA protocols, as judged by PCR-based genotyping. Elution of DNA from the dried sample is a known rate-limiting step in the published Whatman FTA protocol; this method enables the use of each 3-mm punch of sample for several PCR reactions instead of the standard, one PCR reaction per sample punch. This optimized protocol therefore extends the usefulness and cost effectiveness of each buccal swab sample collected, when used for nucleic acid PCR and genotyping.
Keyword Buccal swab
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Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 18:07:25 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience