CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages

Dave, R. K., Hume, D. A., Elsegood, C and Kellie, S (2009) CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages. Experimental Cell Research, 315 10: 1734-1744. doi:10.1016/j.yexcr.2009.02.023


Author Dave, R. K.
Hume, D. A.
Elsegood, C
Kellie, S
Title CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.yexcr.2009.02.023
Open Access Status Not yet assessed
Volume 315
Issue 10
Start page 1734
End page 1744
Total pages 11
Editor U. Lendahl
Place of publication Maryland Heights, MO, U. S. A.
Publisher Academic Press (Elsevier)
Language eng
Subject C1
920108 Immune System and Allergy
110707 Innate Immunity
Abstract In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity.
Keyword Tyrosine phosphatase
Protein-tyrosine-phosphatase
macrophage
Cytoskeleton
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 18:07:03 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience