Circular proteins from melicytus (violaceae) refine the conserved protein and gene architecture of cyclotides

Trabi, M, Mylne, J. S., Sando, L and Craik, D. J. (2009) Circular proteins from melicytus (violaceae) refine the conserved protein and gene architecture of cyclotides. Organic & Biomolecular Chemistry, 7 11: 2378-2388. doi:10.1039/b823020j


Author Trabi, M
Mylne, J. S.
Sando, L
Craik, D. J.
Title Circular proteins from melicytus (violaceae) refine the conserved protein and gene architecture of cyclotides
Journal name Organic & Biomolecular Chemistry   Check publisher's open access policy
ISSN 1477-0520
Publication date 2009-04-06
Year available 2009
Sub-type Article (original research)
DOI 10.1039/b823020j
Open Access Status Not Open Access
Volume 7
Issue 11
Start page 2378
End page 2388
Total pages 11
Editor Dr. Vikki Allen
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract Cyclotides are cyclic disulfide rich mini-proteins found in various Rubiaceae (coffee family), Violaceae (violet family) and Cucurbitaceae (squash family) plant species. Within the Violaceae, cyclotides have been found in numerous species of the genus Viola as well as species from two other genera, namely Hybanthus and Leonia. This is the first in-depth report of cyclotides in the genus Melicytus (Violaceae). We present the chromatographic profiles of extracts of eight Melicytus species and one Melicytus hybrid that were found to contain these circular peptides. We isolated and characterised five novel cyclotides (mra1 to mra5) from the aerial parts of a common New Zealand tree, Melicytus ramiflorus. All five peptides show the characteristics of the bracelet subfamily of cyclotides. Furthermore, we isolated 17 non-redundant cDNA clones from the leaves of Melicytus ramiflorus encoding cyclotide prepropeptides. This detailed report on the presence of cyclotides in several species of the genus Melicytus further strengthens our hypothesis that cyclotides are ubiquitous in Violaceae family plants and provides additional insight into the biochemical processing mechanisms that produce the cyclic protein backbone of this unique family of ultra-stable plant proteins.
Keyword Chemistry, Organic
Chemistry
CHEMISTRY, ORGANIC
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 18:06:09 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience