Crystallization of the flexible nuclear import receptor importin-beta in the unliganded state

Roman, Noelia, Kirkby, Brenda, Marfori, Mary, Kobe, Bostjan and Forwood, Jade K. (2009) Crystallization of the flexible nuclear import receptor importin-beta in the unliganded state. Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online, 65 6: 625-628. doi:10.1107/S1744309109016820

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Author Roman, Noelia
Kirkby, Brenda
Marfori, Mary
Kobe, Bostjan
Forwood, Jade K.
Title Crystallization of the flexible nuclear import receptor importin-beta in the unliganded state
Formatted title
Crystallization of the flexible nuclear import receptor importin-β in the unliganded state
Journal name Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online   Check publisher's open access policy
ISSN 1744-3091
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1107/S1744309109016820
Open Access Status File (Publisher version)
Volume 65
Issue 6
Start page 625
End page 628
Total pages 4
Editor G. Kostorz
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Language eng
Subject C1
060112 Structural Biology (incl. Macromolecular Modelling)
970106 Expanding Knowledge in the Biological Sciences
Formatted abstract
The transport of macromolecules across the nuclear envelope is an essential eukaryotic process that enables proteins such as transcription factors, polymerases and histones to gain access to the genetic material contained within the nucleus. Importin-β plays a central role in the nucleocytoplasmic transport process, mediating nuclear import through a range of interactions with cytoplasmic, nuclear and nuclear pore proteins such as importin-α, Ran, nucleoporins and various cargo molecules. The unliganded form of the full-length yeast importin-β has been expressed and crystallized. The crystals were obtained by vapour diffusion at pH 6.5 and 290 K. The crystals belonged to space group P21 (unit-cell parameters ɑ = 58.17, b = 127.25, c = 68.52 Å, β = 102.23). One molecule is expected in the asymmetric unit. The crystals diffracted to 2.4 Å resolution using a laboratory X-ray source and were suitable for crystal structure determination.
Keyword Structural basis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 03 Sep 2009, 18:03:39 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences