Conventional dendritic cells are the critical donor APC presenting alloantigen after experimental bone marrow transplantation

Markey, KA, Banovic, T, Kuns, RD, Olver, SD, Don, ALJ, Raffelt, NC, Wilson, YA, Raggatt, LJ, Pettit, AR, Bromberg, JS, Hill, GR and MacDonald, KPA (2009) Conventional dendritic cells are the critical donor APC presenting alloantigen after experimental bone marrow transplantation. BLOOD, 113 22: 5644-5649. doi:10.1182/blood-2008-12-191833


Author Markey, KA
Banovic, T
Kuns, RD
Olver, SD
Don, ALJ
Raffelt, NC
Wilson, YA
Raggatt, LJ
Pettit, AR
Bromberg, JS
Hill, GR
MacDonald, KPA
Title Conventional dendritic cells are the critical donor APC presenting alloantigen after experimental bone marrow transplantation
Journal name BLOOD   Check publisher's open access policy
ISSN 0006-4971
Publication date 2009-05-01
Year available 2009
Sub-type Article (original research)
DOI 10.1182/blood-2008-12-191833
Open Access Status DOI
Volume 113
Issue 22
Start page 5644
End page 5649
Total pages 5
Editor Dr. Sanford J. Shattil
Place of publication United States
Publisher American Society of Hematology
Language eng
Subject C1
110702 Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
Abstract We have quantified the relative contribution of donor antigen-presenting cell populations to alloantigen presentation after bone marrow transplantation (BMT) by using transgenic T cells that can respond to host-derived alloantigen presented within the donor major histocompatibility complex. We also used additional transgenic/knockout donor mice and/or monoclonal antibodies that allowed conditional depletion of conventional dendritic cells (cDCs), plasmacytoid DC (pDCs), macrophages, or B cells. Using these systems, we demonstrate that donor cDCs are the critical population presenting alloantigen after BMT, whereas pDCs and macrophages do not make a significant contribution in isolation. In addition, alloantigen presentation was significantly enhanced in the absence of donor B cells, confirming a regulatory role for these cells early after transplantation. These data have major implications for the design of therapeutic strategies post-BMT, and suggest that cDC depletion and the promotion of B-cell reconstitution may be beneficial tools for the control of alloreactivity.
Keyword VERSUS-HOST-DISEASE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Thu, 03 Sep 2009, 18:03:16 EST by Mr Andrew Martlew on behalf of School of Medicine