Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia

Cummins, S. F., Leblanc, L., Degnan, B. M. and Nagle, G. T. (2009) Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia. Journal of Experimental Biology, 212 13: 2037-2044. doi:10.1242/jeb.026427

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Author Cummins, S. F.
Leblanc, L.
Degnan, B. M.
Nagle, G. T.
Title Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia
Formatted title
Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia
Journal name Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
1477-9145
Publication date 2009-07-01
Sub-type Article (original research)
DOI 10.1242/jeb.026427
Open Access Status File (Publisher version)
Volume 212
Issue 13
Start page 2037
End page 2044
Total pages 8
Editor Steve Perry
Hans Hoppeler
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Language eng
Subject C1
060604 Comparative Physiology
830103 Aquaculture Molluscs (excl. Oysters)
Formatted abstract
An ability to sense and respond to environmental cues is essential to the survival of most marine animals. How water-borne chemical cues are detected at the molecular level and processed by molluscs is currently unknown. In this study, we cloned two genes from the marine mollusk Aplysia dactylomela which encode multi-transmembrane proteins. We have performed in situ hybridization that reveals expression and spatial distribution within the long-distance chemosensory organs, the rhinophores. This finding suggests that they could be receptors involved in binding water-borne chemicals and coupling to an intracellular signal pathway. In support of this, we found expression of a phospholipase C and an inositol trisphosphate receptor in the rhinophore sensory epithelia and possibly distributed within outer dendrites of olfactory sensory neurons. In Aplysia, mate attraction and subsequent reproduction is initiated by responding to a cocktail of water-borne protein pheromones released by animal conspecifics. We show that the rhinophore contraction in response to pheromone stimulants is significantly altered following phospholipase C inhibition. Overall, these data provide insight into the molecular components of chemosensory detection in a mollusk. An important next step will be the elucidation of how these coordinate the detection of chemical cues present in the marine environment and activation of sensory neurons.
Keyword Aplysia
Receptor
Pheremones
Phospholiopase C
Inositol trisphosphate receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 17:59:45 EST by Mr Andrew Martlew on behalf of School of Biological Sciences