Genome-wide identification of long noncoding RNAs in CD8(+) T cells

Pang, K. C., Dinger, M. E., Mercer, T. R., Malquori, L, Grimmond, S. M., Chen, W. S. and Mattick, J. S. (2009) Genome-wide identification of long noncoding RNAs in CD8(+) T cells. Journal of Immunology, 182 12: 7738-7748. doi:10.4049/jimmunol.0900603

Author Pang, K. C.
Dinger, M. E.
Mercer, T. R.
Malquori, L
Grimmond, S. M.
Chen, W. S.
Mattick, J. S.
Title Genome-wide identification of long noncoding RNAs in CD8(+) T cells
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.4049/jimmunol.0900603
Open Access Status
Volume 182
Issue 12
Start page 7738
End page 7748
Total pages 11
Editor Jeremy M. Boss
Place of publication Bethesda, MD, U.S.A.
Publisher American Association of Immunologists
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060406 Genetic Immunology
Abstract Previous research into the molecular mechanisms that underlie Ag-specific CD8+ T cell differentiation and function has largely focused on the role of proteins. However, it is now apparent that the mammalian genome expresses large numbers of long (>200 nt) nonprotein-coding RNAs (ncRNAs), and there is increasing evidence that these RNAs have important regulatory functions, particularly in the regulation of epigenetic processes underpinning cell differentiation. In this study, we show that CD8+ T cells express hundreds of long ncRNAs, many of which are lymphoid-specific and/or change dynamically with lymphocyte differentiation or activation. Numerous ncRNAs surround or overlap immunologically important protein-coding genes and can be predicted to function via a range of regulatory mechanisms. The overlap of many long ncRNAs expressed in CD8+ T cells with microRNAs and small interfering RNAs further suggests that long ncRNAs may be processed into and exert their effects via smaller functional species. Finally, we show that the majority of long ncRNAs expressed in CD8+ T cells harbor signatures of evolutionary conservation, secondary structures, and/or regulated promoters, further supporting their functionality. Taken together, our findings represent the first systematic discovery of long ncRNAs expressed in CD8+ T cells and suggest that many of these transcripts are likely to play a role in adaptive immunity.
Keyword Small interfering RNAS
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Created: Thu, 03 Sep 2009, 17:59:35 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience