Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice

Wilhelm, Dagmar, Washburn, Linda L., Truong, Vy, Fellous, Marc, Eicher, EM and Koopman, Peter (2009) Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mechanisms of Development, 126 5-6: 324-336. doi:10.1016/j.mod.2009.02.006

Author Wilhelm, Dagmar
Washburn, Linda L.
Truong, Vy
Fellous, Marc
Eicher, EM
Koopman, Peter
Title Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice
Journal name Mechanisms of Development   Check publisher's open access policy
ISSN 0925-4773
Publication date 2009-05-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.mod.2009.02.006
Open Access Status DOI
Volume 126
Issue 5-6
Start page 324
End page 336
Total pages 13
Editor M. Leptin
Place of publication Clare, Ireland
Publisher Elsevier Ireland
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
Abstract Ovotestis development in B6-XYPOS mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, Rspo1) development in B6-XYPOS ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not up-regulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate Sox9; (2) challenge the established view that SOX9 is responsible for down-regulating Sry expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development.
Keyword Sry
Mammalian sex determination
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DW (HD049431)
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 61 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 17:58:59 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience