Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12

Philip, Daniel S., Sarovich, Derek S. and Pemberton, John M. (2009) Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12. Plasmid, 62 1: 39-43. doi:10.1016/j.plasmid.2009.03.002


Author Philip, Daniel S.
Sarovich, Derek S.
Pemberton, John M.
Title Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12
Formatted title
Complete sequence and analysis of the stability functions of pPSX, a vector that allows stable cloning and expression of Streptomycete genes in Escherichia coli K12
Journal name Plasmid   Check publisher's open access policy
ISSN 0147-619X
Publication date 2009-03-20
Sub-type Article (original research)
DOI 10.1016/j.plasmid.2009.03.002
Open Access Status DOI
Volume 62
Issue 1
Start page 39
End page 43
Total pages 5
Editor D.K. Chattoraj
Place of publication United States
Publisher Academic Press
Language eng
Subject C1
060501 Bacteriology
970106 Expanding Knowledge in the Biological Sciences
Abstract The broad host range, cloning and expression vector pPSX has been completely sequenced and analysed. pPSX is 14.7 kb in length and contains the fusion of two continuous segments of the parental 34 kb, IncW plasmid pR388. pPSX appears to have retained at least three sets of gene/s which contribute in different ways to plasmid stability. The first of these parB, is a known participant in the partitioning of low-copy number plasmids. While the adjoining gene, orf35, has high homology with kfrA, a putative plasmid nucleoid organiser that is often associated with the ParAB family of proteins. The second set of genes; orfs 18, 19, 20, whose exact functions are not clear, have homology to the stability operons of both IncW and IncN plasmids. The third is the resolvase, resP, which may resolve plasmid multimers that can lead to plasmid instability. pPSX is a small, stable cloning vector good for cloning and expression of a wide range of genes, including those from streptomycetes. Crown Copyright
Keyword Streptomyces
E. Coli
Stable vector
Complete sequence
pPSX
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 03 Sep 2009, 17:58:25 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences