Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS

Davis, J, Jones, Al and Lewis, R. J. (2009) Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS. Peptides, 30 7: 1222-1227. doi:10.1016/j.peptides.2009.03.019


Author Davis, J
Jones, Al
Lewis, R. J.
Title Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS
Journal name Peptides   Check publisher's open access policy
ISSN 0196-9781
Publication date 2009-07-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.peptides.2009.03.019
Open Access Status DOI
Volume 30
Issue 7
Start page 1222
End page 1227
Total pages 6
Editor Abba J. Kastin
Place of publication New York, USA
Publisher Elsevier
Language eng
Subject C1
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
970106 Expanding Knowledge in the Biological Sciences
Abstract Cone snails have evolved an assortment of venom peptides as an evolutionary strategy for rapid prey immobilization and defence. Earlier studies estimated not, vert, similar100 conopeptides per species. In this study we optimized liquid chromatography and electrospray ionization mass spectrometry for the detection of conopeptides in crude venom to characterize conopeptides present in the venom of individual specimens of Conus textile, C. imperialis and C. marmoreus. Using this approach, we have expanded the predicted number of venom peptides 10-fold to an estimate of 1000–1900 conopeptides per species. Our investigation has also revealed a surprisingly high level of intra-species variation that distinguishes cone snails from other venomous species including spiders and scorpions. Given this inherent diversity and variability, more sensitive bioassays and sequencing techniques will be required to fully explore conotoxin bioactivity.
Keyword Venom peptide
Mass Spectrometry
Venomics
cone snail
Conotoxin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Sep 2009, 17:49:54 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience