Synaptic proteome changes in the superior frontal gyrus and occipital cortex of the alcoholic brain

Etheridge, Naomi, Lewohl, Joanne M., Mayfield, R. Dayne, Harris, R. Adron and Dodd, Peter R. (2009) Synaptic proteome changes in the superior frontal gyrus and occipital cortex of the alcoholic brain. Proteomics Clinical Applications, 3 6: 730-742. doi:10.1002/prca.200800202

Author Etheridge, Naomi
Lewohl, Joanne M.
Mayfield, R. Dayne
Harris, R. Adron
Dodd, Peter R.
Title Synaptic proteome changes in the superior frontal gyrus and occipital cortex of the alcoholic brain
Journal name Proteomics Clinical Applications   Check publisher's open access policy
ISSN 1862-8346
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1002/prca.200800202
Open Access Status DOI
Volume 3
Issue 6
Start page 730
End page 742
Total pages 13
Editor Michael J. Dunn
Place of publication Germany
Publisher Wiley-VCH Verlag GmbH & Co KGaA
Language eng
Subject C1
060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
060111 Signal Transduction
920414 Substance Abuse
Abstract Cognitive deficits and behavioral changes that result from chronic alcohol abuse are a consequence of neuropathological changes that alter signal transmission through the neural network. To focus on the changes that occur at the point of connection between the neural network cells, synaptosomal preparations from post-mortem human brain of six chronic alcoholics and six non-alcoholic controls were compared using 2-D differential in-gel electrophoresis (DIGE). Functionally affected and spared regions (superior frontal gyrus, SFG, and occipital cortex, OC, respectively) were analyzed from both groups to further investigate the specific pathological response that alcoholism has on the brain. Forty-nine proteins were differentially regulated between the SFG of alcoholics and the SFG of controls and 94 proteins were regulated in the OC with an overlap of 23 proteins. Additionally, the SFG was compared to the OC within each group (alcoholics or controls) to identify region-specific differences. A selection was identified by MALDI-TOF mass spectrometry revealing proteins involved in vesicle transport, metabolism, folding and trafficking, and signal transduction, all of which have the potential to influence synaptic activity. A number of proteins identified in this study have been previously related to alcoholism; however, the focus on synaptic proteins has also uncovered novel alcoholism-affected proteins. Further exploration of these proteins will illuminate the mechanisms altering synaptic plasticity, and thus neuronal signaling and response, in the alcoholic brain.
Keyword Human brain
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID AA12404
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 23 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 17:49:33 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences