Quantification and its applications in fluorescent microscopy imaging

Hamilton, Nicholas (2009) Quantification and its applications in fluorescent microscopy imaging. Traffic, 10 8: 951-961. doi:10.1111/j.1600-0854.2009.00938.x

Author Hamilton, Nicholas
Title Quantification and its applications in fluorescent microscopy imaging
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
Publication date 2009-08-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2009.00938.x
Open Access Status DOI
Volume 10
Issue 8
Start page 951
End page 961
Total pages 11
Editor Mark C. Marsh
Place of publication Malden, MA, U.S.A.
Publisher Wiley - Blackwell
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060102 Bioinformatics
Abstract Fluorescent microscope imaging technologies have developed at a rapid pace in recent years. High-throughput 2D fluorescent imaging platforms are now in wide use and are being applied on a proteome wide scale. Multiple fluorophore 3D imaging of live cells is being used to give detailed localization and subcellular structure information. Further, 2D and 3D video microscopy are giving important insights into the dynamics of protein localization and transport. In parallel with these developments, significant research has gone into developing new methodologies for quantifying and extracting meaning from the imaging data. Here we outline and give entry points to the literature on approaches to quantification such as segmentation, tracking, automated classification and data visualization. Particular attention is paid to the distinction between and application of concrete quantification measures such as number of objects in a cell, and abstract measures such as texture. Copyright © 1999–2011 John Wiley & Sons, Inc. All Rights Reserved.
Keyword Confocal microscopy
Image analysis
Image Quantification
Subcellular imaging
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 48 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 17:49:16 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience