Faecal carriage of CTX-M-15-producing klebsiella pneumoniae in patients with acute gastroenteritis

Muzaheed, Doi, Y., Adams-Haduch, J.M., Shivannavar, C.T., Paterson, D.L. and Gaddad, S.M. (2009) Faecal carriage of CTX-M-15-producing klebsiella pneumoniae in patients with acute gastroenteritis. Indian Journal of Medical Research, 129 5: 599-602.

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Author Muzaheed
Doi, Y.
Adams-Haduch, J.M.
Shivannavar, C.T.
Paterson, D.L.
Gaddad, S.M.
Title Faecal carriage of CTX-M-15-producing klebsiella pneumoniae in patients with acute gastroenteritis
Journal name Indian Journal of Medical Research   Check publisher's open access policy
ISSN 0971-5916
Publication date 2009-05-01
Year available 2009
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 129
Issue 5
Start page 599
End page 602
Total pages 4
Editor K. Satyanarayana
Place of publication New Delhi, India
Publisher Indian Council of Medical Research
Language eng
Subject C1
920109 Infectious Diseases
110309 Infectious Diseases
Abstract Background & objectives: Data on extended-spectrum beta-lactamases (ESBLs) produced by Gram-negative bacteria including Klebsiella pneumoniae especially molecular types of ESBL genes from India are limited. The present study was conducted to investigate the carriage and ESBL contents of multidrug-resistant K. pneumoniae recovered from patients with gastroenteritis in a rural village in southern India.
Formatted abstract
BACKGROUND & OBJECTIVE:
Data on extended-spectrum beta-lactamases (ESBLs) produced by Gram-negative bacteria including Klebsiella pneumoniae especially molecular types of ESBL genes from India are limited. The present study was conducted to investigate the carriage and ESBL contents of multidrug-resistant K. pneumoniae recovered from patients with gastroenteritis in a rural village in southern India.

METHODS:

Nine K. pneumoniae isolates obtained from 45 stool samples from patients with gastroenteritis from one rural and two urban sites, in southern India were included in the study. Antibiotic susceptibility testing, PCR analysis and sequencing were conducted to characterize the ESBL genes. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE).

RESULTS:

All the isolates were found to be resistant to at least one of the third generation cephalosporins tested. All the study isolates were confirmed to produce ESBLs. PCR and sequencing revealed the responsible gene to be bla(CTX-M-15). bla(TEM) and bla(SHV) were absent. PFGE indicated that fi ve of seven isolates from villagers were genetically closely related, and in turn were related to isolates from patients in two urban areas in this region.

INTERPRETATION & CONCLUSION:
Our findings showed that genetically-related isolates of K. pneumoniae producing CTX-M-15 were present in multiple areas in southern India. Larger studies need to be done in various geographical regions of the country to better define the molecular epidemiology of ESBL-producing K. pneumoniae and its clinical implications.
Keyword CTX-M-15
Extended spectrum ß-lactamases
Klebsiella pneumoniae
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes No DOI

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
2010 Higher Education Research Data Collection
 
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Created: Thu, 03 Sep 2009, 17:47:45 EST by Mr Andrew Martlew on behalf of UQ Centre for Clinical Research