Modification and optimization of organosilica microspheres for peptide synthesis and microsphere-based immunoassays

Surawski, PPT, Battersby, BJ, Vogel, R, Lawrie, G and Trau, M (2009) Modification and optimization of organosilica microspheres for peptide synthesis and microsphere-based immunoassays. Molecular Biosystems, 5 8: 826-831. doi:10.1039/b817080k


Author Surawski, PPT
Battersby, BJ
Vogel, R
Lawrie, G
Trau, M
Title Modification and optimization of organosilica microspheres for peptide synthesis and microsphere-based immunoassays
Journal name Molecular Biosystems   Check publisher's open access policy
ISSN 1742-206X
1742-2051
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1039/b817080k
Open Access Status Not Open Access
Volume 5
Issue 8
Start page 826
End page 831
Total pages 6
Editor Sarah Thomas
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
A new generation of optically encoded organosilica microspheres, suitable for both solid phase synthesis and multiplexed microsphere-based assays, has recently been described. One of the challenges of producing this type of dual-purpose solid support is that the particles must maintain their morphology as well as their encoding during exposure to the solvents used for solid phase synthesis. In this article, organosilica microspheres are subjected to ammonia treatment methods for enhancing the condensation of the silica matrix and their subsequent resilience toward organic solvents and peptide synthesis reagents is described. The instability of the untreated supports toward organic synthesis reagents was found to be associated with the swelling and permeability of these microspheres in organic solvents. Post-synthesis ammonia treatment resulted in reduced permeability, as demonstrated by dye uptake studies. The treated microspheres exhibited enhanced stability against organic synthesis conditions and were characterized via a variety of techniques including electron microscopy, 29 Si-nuclear magnetic resonance (NMR) and optical microscopy. The ammonia-treated supports were subjected to an Fmoc peptide synthesis procedure and successfully applied in a model microsphere-based flow cytometric immunoassay. © The Royal Society of Chemistry 2009.
Keyword MOLECULAR-SIEVES
Biochemistry and Molecular Biophysics
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 17:47:11 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology