Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization

Jensen, Paul D., Hardin, Matthew T. and Clarke, William P. (2009) Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization. Bioresource Technology, 100 21: 5219-5225. doi:10.1016/j.biortech.2009.05.018


Author Jensen, Paul D.
Hardin, Matthew T.
Clarke, William P.
Title Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization
Journal name Bioresource Technology   Check publisher's open access policy
ISSN 0960-8524
1873-2976
Publication date 2009-11-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.biortech.2009.05.018
Open Access Status
Volume 100
Issue 21
Start page 5219
End page 5225
Total pages 7
Editor A. Pandey
C. Larroche
S. C. Ricke
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject C1
Abstract This study determines cellulose solubilization kinetics from controlled batch digestions and shows the effect of inoculum biomass concentrations. Separate measurements and analyzes were performed for sessile biomass (biofilms) and planktonic biomass (free suspensions). Experiments were conducted using either leachate enriched on cellulose or rumen fluid as inoculum to assess if the effect of biomass concentration was consistent for microbial populations from different source environments. All batch digestions were fitted to a first-order kinetic model (R-2 ranging from 0.94 to 0.99). Regression analysis used to compare the first-order hydrolysis rate showed that the first-order hydrolysis rate was most strongly correlated with the concentration of sessile biomass rather than with the concentration of total or planktonic biomass. The correlation between solubilization rate and sessile biomass was statistically the same for the rumen and leachate inoculated reactors indicating that at low concentration ratios of inoculum to cellulose, the rate of cellulose solubilization is dependant primarily on sessile biomass concentration rather than the species profile of the cellulolytic community.
Keyword Anaerobic digestion
Cellulose
Leachate
Rumen
Hydrolysis rate
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 03 Sep 2009, 17:44:33 EST by Mr Andrew Martlew on behalf of Advanced Water Management Centre