Comparison of methods for characterizing nonideal solute self-association by sedimentation equilibrium

Scott, David J. and Winzor, Donald J. (2009) Comparison of methods for characterizing nonideal solute self-association by sedimentation equilibrium. Biophysical Journal, 97 3: 886-896. doi:10.1016/j.bpj.2009.05.028


Author Scott, David J.
Winzor, Donald J.
Title Comparison of methods for characterizing nonideal solute self-association by sedimentation equilibrium
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
1542-0086
Publication date 2009-08-01
Sub-type Article (original research)
DOI 10.1016/j.bpj.2009.05.028
Open Access Status Not yet assessed
Volume 97
Issue 3
Start page 886
End page 896
Total pages 11
Place of publication St. Louis, MO, United States
Publisher Cell Press
Language eng
Subject 1304 Biophysics
Abstract We have examined in detail analytical solutions of expressions for sedimentation equilibrium in the analytical ultracentrifuge to describe self-association under nonideal conditions. We find that those containing the radial dependence of total solute concentration that incorporate the Adams-Fujita assumption for composition-dependence of activity coefficients reveal potential shortcomings for characterizing such systems. Similar deficiencies are shown in the use of the NONLIN software incorporating the same assumption about the interrelationship between activity coefficients for monomer and polymer species. These difficulties can be overcome by iterative analyses incorporating expressions for the composition-dependence of activity coefficients predicted by excluded volume considerations. A recommendation is therefore made for the replacement of current software packages by programs that incorporate rigorous statistical-mechanical allowance for thermodynamic nonideality in sedimentation equilibrium distributions reflecting solute self-association.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Thu, 03 Sep 2009, 17:42:55 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences