Differential Participation of Angiotensin II Type 1 and 2 Receptors in the Regulation of Cardiac Cell Death Triggered by Angiotensin II

Aranguiz-Urroz, Pablo, Soto, Dagoberto, Contreras, Ariel, Troncoso, Rodrigo, Chiong, Mario, Montenegro, Jose, Venegas, Daniel, Smolic, Christian, Ayala, Pedro, Thomas, Walter G., Lavandero, Sergio and Diaz-Araya, Guillermo (2009) Differential Participation of Angiotensin II Type 1 and 2 Receptors in the Regulation of Cardiac Cell Death Triggered by Angiotensin II. American Journal of Hypertension, 22 5: 569-576. doi:10.1038/ajh.2009.32


Author Aranguiz-Urroz, Pablo
Soto, Dagoberto
Contreras, Ariel
Troncoso, Rodrigo
Chiong, Mario
Montenegro, Jose
Venegas, Daniel
Smolic, Christian
Ayala, Pedro
Thomas, Walter G.
Lavandero, Sergio
Diaz-Araya, Guillermo
Title Differential Participation of Angiotensin II Type 1 and 2 Receptors in the Regulation of Cardiac Cell Death Triggered by Angiotensin II
Journal name American Journal of Hypertension   Check publisher's open access policy
ISSN 0895-7061
Publication date 2009-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1038/ajh.2009.32
Open Access Status Not yet assessed
Volume 22
Issue 5
Start page 569
End page 576
Total pages 8
Editor M H Alderman
Place of publication United Staes
Publisher American Journal of Hypertension
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060110 Receptors and Membrane Biology
060111 Signal Transduction
060199 Biochemistry and Cell Biology not elsewhere classified
060108 Protein Trafficking
060602 Animal Physiology - Cell
110201 Cardiology (incl. Cardiovascular Diseases)
110903 Central Nervous System
Abstract Background: The Angiotensin II (Ang II) type 1 (AT"1R) and type 2 (AT"2R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.MethodsWe ectopically expressed AT"1R and AT"2R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.ResultsIn adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT"1R, and no effect of Ang II on cell viability was observed following AT"1R or AT"2R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT"1R (but not the AT"2R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.ConclusionsWe conclude that Ang II/AT"1R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca influx.
Formatted abstract
BACKGROUND The Angiotensin II (Ang II) type 1 (AT(1)R) and type 2 (AT(2)R) receptors are increased in the heart following myocardial infarction and dilated cardiomyopathy, yet their contribution at a cellular level to compensation and/or failure remains controversial.

METHODS We ectopically expressed AT(1)R and AT(2)R in cultured adult rat cardiomyocytes and cardiac fibroblasts to investigate Ang II-mediated cardiomyocyte hypertrophy and cardiac cell viability.

RESULTS In adult rat cardiomyocytes, Ang II did not induce hypertrophy via the AT(1)R, and no effect of Ang II on cell viability was observed following AT(1)R or AT(2)R expression. In adult rat cardiac fibroblasts, Ang II stimulated cell death by apoptosis via the AT(1)R (but not the AT(2)R), which required the presence of extracellular calcium, and induced a rapid dissipation of mitochondrial membrane potential, which was significant from 8 h.

CONCLUSIONS We conclude that Ang II/AT(1)R triggers apoptosis in adult rat cardiac fibroblasts, which is dependent on Ca2+ influx.
Keyword Peripheral Vascular Disease
Cardiovascular System & Cardiology
PERIPHERAL VASCULAR DISEASE
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 1061059
15010006
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Wed, 02 Sep 2009, 20:49:00 EST by Cameron Harris on behalf of School of Biomedical Sciences