Development and Optimization of MicroRNA against Relaxin-3

Callander, G. E., Thomas, W. G. and Bathgate, R. A. D. (2009) Development and Optimization of MicroRNA against Relaxin-3. Annals of the New York Academy of Sciences, 1160 Relaxin and Related Peptides: Fifth International Conference: 261-264. doi:10.1111/j.1749-6632.2008.03816.x


Author Callander, G. E.
Thomas, W. G.
Bathgate, R. A. D.
Title Development and Optimization of MicroRNA against Relaxin-3
Journal name Annals of the New York Academy of Sciences   Check publisher's open access policy
ISSN 0077-8923
ISBN 978-1-57331-721-4
Publication date 2009-04-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1749-6632.2008.03816.x
Volume 1160
Issue Relaxin and Related Peptides: Fifth International Conference
Start page 261
End page 264
Total pages 4
Editor Douglas Braaten
Place of publication United States
Publisher Wiley-Blackwell Publishing
Language eng
Subject 060110 Receptors and Membrane Biology
060111 Signal Transduction
060199 Biochemistry and Cell Biology not elsewhere classified
060108 Protein Trafficking
060102 Bioinformatics
110201 Cardiology (incl. Cardiovascular Diseases)
110903 Central Nervous System
970106 Expanding Knowledge in the Biological Sciences
C1
Abstract Recent in vivo studies suggest a role for relaxin-3 in feeding and stress. To further elucidate the function of relaxin-3 in the central nervous system, we have employed a complementary approach, based on RNA interference, to modulate relaxin-3 expression. We have designed, constructed, and characterized three microRNAs (miRNAs) targeting different regions of the relaxin-3 transcript. These were tested to determine the amount of miRNA required to achieve the greatest knockdown and for their ability to reduce the expression of relaxin-3 in transfected HEK293Tcells. All miRNA constructs significantly reduced relaxin-3-induced cAMP responses; however, miR499 was most effective. This should be a useful tool for in vitro studies and central targeting of relaxin-3 in vivo using viral delivery systems.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Tue, 01 Sep 2009, 22:44:54 EST by Cameron Harris on behalf of School of Biomedical Sciences