Analysing persistent organic pollutants in eggs, blood and tissue of the green sea turtle (Chelonia mydas) using gas chromatography with tandem mass spectrometry (GC-MS/MS)

van de Merwe, Jason Paul, Hodge, Mary, Whittier, Joan Margaret and Lee, Shing Yip (2009) Analysing persistent organic pollutants in eggs, blood and tissue of the green sea turtle (Chelonia mydas) using gas chromatography with tandem mass spectrometry (GC-MS/MS). Analytical and Bioanalytical Chemistry, 393 6-7: 1719-1731. doi:10.1007/s00216-009-2608-0


Author van de Merwe, Jason Paul
Hodge, Mary
Whittier, Joan Margaret
Lee, Shing Yip
Title Analysing persistent organic pollutants in eggs, blood and tissue of the green sea turtle (Chelonia mydas) using gas chromatography with tandem mass spectrometry (GC-MS/MS)
Journal name Analytical and Bioanalytical Chemistry   Check publisher's open access policy
ISSN 1618-2642
Publication date 2009-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s00216-009-2608-0
Volume 393
Issue 6-7
Start page 1719
End page 1731
Total pages 13
Editor Christina E Dyllick
Place of publication Germany
Publisher Springer
Language eng
Subject C1
060806 Animal Physiological Ecology
970106 Expanding Knowledge in the Biological Sciences
Abstract Investigation into persistent organic pollutants (POPs) in sea turtles is an important area of conservation research due to the harmful effects of these chemicals. However, the analysis of POPs in the green sea turtle (Chelonia mydas) has been limited by methods with relatively high limits of detection and high costs associated with multiple sample injections into complex arrangements of analytical equipment. The present study aimed to develop a method that could detect a large number of POPs in the blood, eggs and tissue of C. mydas at trace concentrations. A gas chromatography with tandem mass spectrometry (GC-MS/MS) method was developed that could report 125 POP compounds to a limit of detection of <35 pg g(-1) using a single sample injection. The recoveries of internal standards ranged from 30% to 96%, and the standard reference materials were reported to within 70% of the certified values. The coefficient of variation of ten replicates of pooled egg sample was <20% for all compounds, indicating low within-run variation. This GC-MS/MS method is an improvement of previous methods for analysing POPs in C. mydas in that more compounds can be reported at lower concentrations and the accuracy and precision of the method are sound. This is particularly important for C. mydas as they occupy a low trophic level and have lower concentrations of POPs. This method is also simple to set up, and there are minimal differences in sample preparation for the different tissue types.
Keyword Chelonia mydas
Persistent organic pollutants
GC-MS/MS
Chlorinated and brominated compounds
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Fri, 28 Aug 2009, 22:42:12 EST by Shirley Rey on behalf of School of Biomedical Sciences