Adiponectin Regulate Growth Hormone Secretion Via Adiponectin Receptor Mediated Ca2+ Signalling in Rat Somatotrophs In Vitro

Steyn, F. J., Boehme, F., Vargas, E., Wang, K., Parkington, H. C., Rao, J. R. and Chen, C. (2009) Adiponectin Regulate Growth Hormone Secretion Via Adiponectin Receptor Mediated Ca2+ Signalling in Rat Somatotrophs In Vitro. Journal of Neuroendocrinology, 21 8: 698-704. doi:10.1111/j.1365-2826.2009.01887.x


Author Steyn, F. J.
Boehme, F.
Vargas, E.
Wang, K.
Parkington, H. C.
Rao, J. R.
Chen, C.
Title Adiponectin Regulate Growth Hormone Secretion Via Adiponectin Receptor Mediated Ca2+ Signalling in Rat Somatotrophs In Vitro
Journal name Journal of Neuroendocrinology   Check publisher's open access policy
ISSN 0953-8194
Publication date 2009-08-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1365-2826.2009.01887.x
Open Access Status Not yet assessed
Volume 21
Issue 8
Start page 698
End page 704
Total pages 7
Editor David R Grattan
Place of publication Malden, MA, USA
Publisher Wiley- Blackwell Publishing Ltd.
Language eng
Subject C1
920507 Women's Health
060110 Receptors and Membrane Biology
060602 Animal Physiology - Cell
060603 Animal Physiology - Systems
Abstract Obesity is associated with reduced levels of growth hormone (GH) and the disruption of pulsatile GH secretion. This results in relative GH deficiency. It is likely that a regulatory relationship between GH secretion and adipose tissue exists as the secretion of GH recovers to normal levels after a reduction in body weight. This report characterise the expression and interaction of adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) and adiponectin, respectively, in regulating the activity of GH secreting cells. Polymerase chain reaction analysis of the GH3 cell line, rat anterior pituitary gland and isolated somatotroph cells from transgenic GFP expressing mice confirmed the expression of both AdipoR1 and AdipoR2 in GH secretory cells. Because GH cells expressed both receptors, it is likely that the measured increase in GH secretion, observed in primary cultured rat pituitary cells after 30 min of incubation with full-length murine adiponectin, was mediated by a direct receptor regulated process. Adiponectin induced an increase in intracellular Ca2+ through both the influx of extracellular Ca2+ and the release of intracellular Ca2+ stores resulting in the secretion of GH. Furthermore, results confirm that this increase in GH secretion depended mainly on an increase in Ca2+ influx through L-type Ca2+ channels. It is concluded that adiponectin directly regulates GH secretion from somatotrophs by binding to either adiponectin receptor, and that this is mediated via a similar process observed after the stimulation of GH secretion by GH-releasing hormone.
Keyword obesity
growth hormone
adiponectin
calcium
receptors
membrane
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Fri, 28 Aug 2009, 22:29:31 EST by Shirley Rey on behalf of School of Biomedical Sciences