An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay

Athanassiadis, B, Abbott, PV, George, N and Walsh, LJ (2009) An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay. Australian Dental Journal, 54 2: 141-146. doi:10.1111/j.1834-7819.2009.01107.x


Author Athanassiadis, B
Abbott, PV
George, N
Walsh, LJ
Title An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay
Formatted title
An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay
Journal name Australian Dental Journal   Check publisher's open access policy
ISSN 0045-0421
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1834-7819.2009.01107.x
Open Access Status Not yet assessed
Volume 54
Issue 2
Start page 141
End page 146
Total pages 6
Place of publication Sydney
Publisher Australian Dental Assoc Inc.
Language eng
Subject 920113 Oro-Dental Disorders
C1
110503 Endodontics
Abstract The aim of this study was to determine the in vitro antimicrobial activities of various endodontic medicaments and their bases against selected organisms using an agar diffusion assay.
Formatted abstract
Background:
The aim of this study was to determine the in vitro antimicrobial activities of various endodontic medicaments and their bases against selected organisms using an agar diffusion assay.

Methods:
An agar well diffusion assay was used to test the antimicrobial action of some commonly used endodontic medicaments (Ledermix paste, Pulpdent paste, Ultracal paste, and a 50:50 mix of Ledermix and Pulpdent pastes) and their bases. Three bacterial species (E. faecalis, P. micros, P. intermedia) and one yeast (C. albicans) were selected. The diameters of growth inhibition zones and pH were assessed.

Results:
P. micros demonstrated the highest level of in vitro resistance. Pulpdent and Ultracal pastes had the highest pH (12.64 and 12.53, respectively). The addition of Pulpdent to Ledermix did not increase the zone sizes significantly.

Conclusions:
All the commercial products showed some in vitro antimicrobial activity. Ledermix paste and the 50:50 Ledermix/Pulpdent mixture being the most effective in this model. The known anti-inflammatory/analgesic properties of Ledermix and the results from this agar model suggest that the 50:50 Ledermix/Pulpdent combination would be the preferred medicament for clinical use in symptomatic cases, even though the addition of calcium hydroxide to Ledermix did not appear to be synergistic in terms of enhancing the antimicrobial action.
Keyword Endodontics
medicaments
antimicrobial actions
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Dentistry Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
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Created: Sat, 15 Aug 2009, 22:48:25 EST by Margot Dallinger on behalf of School of Dentistry