Delivery of bioactive macromolecules from microporous polymer matrices: Release and activity profiles of lysozyme, collagenase and catalase

Wang, Yiwei, Chang, Hsin-I., Li, Xiongwei, Alpar, Oyar and Coombes, Allan G. A. (2009) Delivery of bioactive macromolecules from microporous polymer matrices: Release and activity profiles of lysozyme, collagenase and catalase. European Journal of Pharmaceutical Sciences, 37 3-4: 387-394. doi:10.1016/j.ejps.2009.03.010


Author Wang, Yiwei
Chang, Hsin-I.
Li, Xiongwei
Alpar, Oyar
Coombes, Allan G. A.
Title Delivery of bioactive macromolecules from microporous polymer matrices: Release and activity profiles of lysozyme, collagenase and catalase
Journal name European Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 0928-0987
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1016/j.ejps.2009.03.010
Volume 37
Issue 3-4
Start page 387
End page 394
Total pages 8
Editor Urtti, A.
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
111504 Pharmaceutical Sciences
8608 Human Pharmaceutical Products
Abstract Microporous polycaprolactone (PCL) matrices containing lysozyme, collagenase and catalase respectively with molecular weight covering a wide range from 14.3 to 240 kDa were produced by a novel method involving rapid cooling of particle suspensions in dry ice. The enzyme loading efficiency (lysozyme (50%), collagenase (75%) and catalase (90%)) depended on the enzyme molecular weight and the non-solvent used to extract acetone from the hardened matrices. Sustained enzyme release occurred from the PCL matrices over 11 days with retained activity dependent on the particular enzyme used (collagenase 100% activity at 11 days, lysozyme 75–80% at 11 days, catalase 10–20% at 5 days). The present findings confirm the potential of microporous PCL matrices for delivering bioactive macromolecules from implantable/insertable depot-type formulations and tissue engineering scaffolds and recommend catalase as a challenging model protein for evaluating such devices.
Keyword polycaprolactone
Enzyme Activity
Catalase
collagenase
Lysozyme
Matrix device lysozyme
Enzymes
Scaffolds
tissue engineering
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 04 Aug 2009, 19:42:03 EST by Charna Kovacevic on behalf of School of Pharmacy