Nitrogen affects cluster root formation and expression of putative peptide transporters

Paungfoo-Lonhienne, Chanyarat, Schenk, Peer M., Lonhienne, Thierry G. A., Brackin, Richard, Meier, Stefan, Rentsch, Doris and Schmidt, Susanne (2009) Nitrogen affects cluster root formation and expression of putative peptide transporters. Journal of Experimental Botany, 60 9: 2665-2676. doi:10.1093/jxb/erp111


Author Paungfoo-Lonhienne, Chanyarat
Schenk, Peer M.
Lonhienne, Thierry G. A.
Brackin, Richard
Meier, Stefan
Rentsch, Doris
Schmidt, Susanne
Title Nitrogen affects cluster root formation and expression of putative peptide transporters
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 1460-2431
0022-0957
Publication date 2009-07-01
Year available 2009
Sub-type Article (original research)
DOI 10.1093/jxb/erp111
Open Access Status DOI
Volume 60
Issue 9
Start page 2665
End page 2676
Total pages 12
Editor Julian A. T Dow
Steve Perry
Hans Hoppeler
Place of publication Oxford, U.K.
Publisher Oxford University Press
Language eng
Subject C1
829899 Environmentally Sustainable Plant Production not elsewhere classified
060705 Plant Physiology
Abstract Non-mycorrhizal Hakea actites (Proteaceae) grows in heathland where organic nitrogen (ON) dominates the soil nitrogen (N) pool. Hakea actites uses ON for growth, but the role of cluster roots in ON acquisition is unknown. The aim of the present study was to ascertain how N form and concentration affect cluster root formation and expression of peptide transporters. Hydroponically grown plants produced most biomass with low molecular weight ON> inorganic N>high molecular weight ON, while cluster roots were formed in the order no-N>ON>inorganic N. Intact dipeptide was transported into roots and metabolized, suggesting a role for the peptide transporter (PTR) for uptake and transport of peptides. HaPTR4, a member of subgroup II of the NRT1/PTR transporter family, which contains most characterized di- and tripeptide transporters in plants, facilitated transport of di- and tripeptides when expressed in yeast. No transport activity was demonstrated for HaPTR5 and HaPTR12, most similar to less well characterized transporters in subgroup III. The results provide further evidence that subgroup II of the NRT1/PTR family contains functional di- and tripeptide transporters. Green fluorescent protein fusion proteins of HaPTR4 and HaPTR12 localized to tonoplast, and plasma- and endomembranes, respectively, while HaPTR5 localized to vesicles of unknown identity. Grown in heathland or hydroponic culture with limiting N supply or starved of nutrients, HaPTR genes had the highest expression in cluster roots and non-cluster roots, and leaf expression increased upon re-supply of ON. It is concluded that formation of cluster roots and expression of PTR are regulated in response to N supply.
Keyword Cluster root
Heathland
Organic nitrogen
Peptide transporters
Proteaceae
Roots
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP0452559
SNF3100A0-107507
Institutional Status UQ

 
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Created: Fri, 10 Jul 2009, 20:25:47 EST by Gail Walter on behalf of School of Biological Sciences