Complement factors C3a and C5a have distinct hemodynamic effects in the rat

Proctor, Lavinia M., Moore, Tyson A., Monk, Peter N., Sanderson, Sam D., Taylor, Stephen M. and Woodruff, Trent M. (2009) Complement factors C3a and C5a have distinct hemodynamic effects in the rat. International Immunopharmacology, 9 6: 800-806. doi:10.1016/j.intimp.2009.03.002


Author Proctor, Lavinia M.
Moore, Tyson A.
Monk, Peter N.
Sanderson, Sam D.
Taylor, Stephen M.
Woodruff, Trent M.
Title Complement factors C3a and C5a have distinct hemodynamic effects in the rat
Journal name International Immunopharmacology   Check publisher's open access policy
ISSN 1567-5769
1878-1705
Publication date 2009-06-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.intimp.2009.03.002
Volume 9
Issue 6
Start page 800
End page 806
Total pages 7
Editor J. E. Talmadge
D. E. Hugli
Place of publication Amsterdam, Netherlands
Publisher Elsevier Science
Language eng
Subject C1
060804 Animal Immunology
970106 Expanding Knowledge in the Biological Sciences
920203 Diagnostic Methods
Abstract In the rat, C5a infusion mediates well-defined effects including hypotension and neutropenia. Conversely, the comparative effect of C3a in the rat is not yet defined. In the current study, we have investigated C3a receptor (C3aR) activation in the rat, using recombinant human C3a, the C3aR agonist WWGKKYRASKLGLAR, which is a C-terminal analogue of C3a, and a nonpeptide C3aR antagonist SB-290157, as pharmacological tools. In vitro, C3a and WWGKKYRASKLGLAR selectively bound to C3aRs and induced degranulation of C3aR-transfected RBL-2H3 cells. C3a or WWGKKYRASKLGLAR-induced degranulation was dose-dependently antagonized in a surmountable fashion by the nonpeptide C3aR antagonist. Intravenous infusion of C3a and WWGKKYRASKLGLAR to rats induced a rapid, transient and concentration-dependent hypertensive response, which was mediated by C3aR-induced prostanoid release. C3a and WWGKKYRASKLGLAR caused a small drop in circulating neutrophils, but a rise in circulating neutrophils was evident after 90–120 min. In contrast to C3a, C5a infusion resulted in hypotension, and rapid and transient neutropenia. These results demonstrate that C3a and C5a mediate distinct effects on blood pressure and circulating polymorphonuclear leukocytes in the rat.
Keyword Complement; C3a
C5a
C3a agonist
Blood pressure
Polymorphonuclear leukocytes
Rat
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Available online 12 March 2009. Issue title: Trends Immusupression,[sic] Berlin, 2008

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Wed, 08 Jul 2009, 01:47:22 EST by Shirley Rey on behalf of School of Biomedical Sciences