Molecular ecology of a facultative swine waste lagoon.

Goh, S. H. M., Mabbett, A. N., Welch, J. P., Hall, S. J. and McEwan, A. G. (2009) Molecular ecology of a facultative swine waste lagoon.. Letters in Applied Microbiology, 48 4: 486-492. doi:10.1111/j.1472-765X.2009.02560.x

Author Goh, S. H. M.
Mabbett, A. N.
Welch, J. P.
Hall, S. J.
McEwan, A. G.
Title Molecular ecology of a facultative swine waste lagoon.
Journal name Letters in Applied Microbiology   Check publisher's open access policy
ISSN 0266-8254
Publication date 2009-04-01
Sub-type Article (original research)
DOI 10.1111/j.1472-765X.2009.02560.x
Open Access Status
Volume 48
Issue 4
Start page 486
End page 492
Total pages 7
Editor Jean-Yves Maillard
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject C1
060299 Ecology not elsewhere classified
960912 Urban and Industrial Water Management
Formatted abstract
Aims: To investigate the microbial ecology of three facultative swine waste lagoons.

Methods and Results:
Phylogenetic analysis of sequences in a 16S rRNA gene clone library and fluorescence in situ hybridization (FISH) analyses were used to assess bacterial diversity in a swine waste lagoon. FISH analysis and Gram-staining were used to compare the microbial communities of all three swine waste lagoons. Six operational taxonomic units were in high relative abundance and corresponded to the following phylotypes; Thiolamprovum, Verrucomicrobia, Acholeplasma, Turicibacter, Clostridium and Bacteroides. PCR was employed to detect the genes apsA and dsrAB which encode for enzymes specifically associated with dissimilatory sulfate-reduction within sulfate-reducing bacteria (SRB). Amplification of these genes confirmed their presence within the lagoons.

Conclusions: All lagoons were dominated by purple sulfur bacteria, affiliated to Thiolamprovum pedioforme. The molecular identification of fermentative bacteria and SRB indicate the following metabolic processes within such facultative ponds: sulfur-cycling, fermentation, inter-species hydrogen transfer and carbon cycling.

Significance and Impact of the Study:
This study provides the first molecular evidence for the existence of a sulfur cycle which is linked to phototrophic sulfide oxidation by purple bacteria and organotrophic sulfate-reduction by SRB.
Keyword facultative lagoons
sulfide oxidation
sulfur cycle
Thiolamprovum pedioforme
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published Online: 23 Feb 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
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Created: Tue, 23 Jun 2009, 23:11:57 EST by Sarah Borsellino on behalf of Advanced Water Management Centre