Divergent expression changes of telomerase and E6/E7 mRNA, following integration of human papillomavirus type 33 in cultured epithelial cells

Peitsaro, Panu, Ruutu, Merja, Syrjänen, Stina and Johansson, Bo (2004) Divergent expression changes of telomerase and E6/E7 mRNA, following integration of human papillomavirus type 33 in cultured epithelial cells. Scandinavian journal of infectious diseases, 36 4: 302-304. doi:10.1080/00365540410027139


Author Peitsaro, Panu
Ruutu, Merja
Syrjänen, Stina
Johansson, Bo
Title Divergent expression changes of telomerase and E6/E7 mRNA, following integration of human papillomavirus type 33 in cultured epithelial cells
Journal name Scandinavian journal of infectious diseases   Check publisher's open access policy
ISSN 0036-5548
Publication date 2004-04-01
Sub-type Article (original research)
DOI 10.1080/00365540410027139
Open Access Status
Volume 36
Issue 4
Start page 302
End page 304
Total pages 3
Place of publication Oslo
Publisher Scandinavian University Press
Language eng
Subject 060506 Virology
Abstract Expression of the viral oncogenes E6 and E7, and telomerase, was investigated, using a cell line from a mild dysplastic vaginal lesion containing human papillomavirus (HPV) type 33. During passaging of the cells, there was a change towards a cancer phenotype, and a shift from episomal to integrated HPV. Levels of hTERT (catalytic subunit of telomerase) mRNA, and telomerase activity in cells carrying episomal virus seemed constant during passaging. During passaging of cells containing integrated HPV, however, the levels of oncogene mRNA decreased, while hTERT mRNA and telomerase activity increased sharply. Thus, in those cells there is no direct correlation between changes of oncogene and telomerase expression. Conceivably, viral oncogene expression might trigger telomerase up-regulation in an early subpopulation of cells, which during subsequent passaging could be selected for.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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