Effect of Confluence State and Passaging to Global Cancer Gene Expression Pattern in Oral Carcinoma Cell Lines

Ruutu, M., Johansson, B., Grenman, R., Syranen, K. and Syranen, S. (2004) Effect of Confluence State and Passaging to Global Cancer Gene Expression Pattern in Oral Carcinoma Cell Lines. Anticancer Research, 24 5: 2627-2631.

Author Ruutu, M.
Johansson, B.
Grenman, R.
Syranen, K.
Syranen, S.
Title Effect of Confluence State and Passaging to Global Cancer Gene Expression Pattern in Oral Carcinoma Cell Lines
Journal name Anticancer Research   Check publisher's open access policy
ISSN 0250-7005
Publication date 2004-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 24
Issue 5
Start page 2627
End page 2631
Total pages 5
Place of publication Attiki, Greece
Publisher International Institute of Anticancer Research
Language eng
Subject 111201 Cancer Cell Biology
Formatted abstract
Background:
After establishment of a cell culture, inhomogenous growth and cell selection takes place at early passages. Reproducing the same confluence state among experiments might be difficult. Materials and

Methods:
Here we used cDNA arrays to compare the global gene expression pattern of two oral cancer cell lines at 80%, 100% and superconfluence stages. Also, the stability of the global gene expression pattern during culture was assessed in two cell lines at passages 10 and 53 (early and late passage). An intraclass correlation coefficient test was used for comparisons.

Results:
The consistency between the different confluence states was almost perfect (> 0.8) and substantial (0.6-0.8). Also the consistency between early and late passages was almost perfect in the cell lines used.

Conclusion:
Our results indicate that neither the state of confluence nor passage have significant effects on the global gene expression profile, despite the variability in expression levels of individual genes.
Keyword Malignant tumor
Stomatology
Oral cavity disease
Oral cancer
Cancerology
Established cell line
Gene expression
Oral cavity
Confluence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
 
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