Analysis of microparticle penetration into human and porcine skin: non-invasive imaging with multiphoton excitation microscopy

Mulholland, William J., Kendall, Mark A., Bellhouse, Brian J. and White, Nick (2002). Analysis of microparticle penetration into human and porcine skin: non-invasive imaging with multiphoton excitation microscopy. In: SPIE: Multiphoton Microscopy in the Biomedical Sciences II. Multiphoton Microscopy in the Biomedical Sciences II, San Jose, CA, USA, (113-122). 20 January 2002. doi:10.1117/12.470685

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Author Mulholland, William J.
Kendall, Mark A.
Bellhouse, Brian J.
White, Nick
Title of paper Analysis of microparticle penetration into human and porcine skin: non-invasive imaging with multiphoton excitation microscopy
Conference name Multiphoton Microscopy in the Biomedical Sciences II
Conference location San Jose, CA, USA
Conference dates 20 January 2002
Proceedings title SPIE: Multiphoton Microscopy in the Biomedical Sciences II   Check publisher's open access policy
Journal name Multiphoton Microscopy in the Biomedical Sciences II   Check publisher's open access policy
Place of Publication Redondo Beach, CA United States
Publisher Society of Photo-optical Instrumentation Engineers
Publication Year 2002
Sub-type Fully published paper
DOI 10.1117/12.470685
Open Access Status File (Publisher version)
ISBN 0-8194-4359-X
ISSN 0277-786X
Volume 4620
Start page 113
End page 122
Total pages 10
Language eng
Abstract/Summary At the University of Oxford and PowderJect Pharmaceuticals plc, a unique form of needle-free injection technology has been developed. Powdered vaccines and drugs in micro-particle form are accelerated in a high-speed gas flow to sufficient velocity to enter the skin, subsequently achieving a pharmaceutical effect. To optimize the delivery of vaccines and drugs with this method a detailed understanding of the interactive processes that occur between the microparticles and the skin is necessary. Investigations to date of micro-particle delivery into excised human and animal tissue have involved image analyses of histology sections. In the present study, a series of investigations were conducted on excised human and porcine skin using the technique of Multi-Photon fluorescence excitation Microscopy (MPM) to image particles and skin structures post-penetration. Micro-particles of various size and composition were imaged with infrared laser excitation. Three-dimensional images of stratum corneum and epidermal cell deformation due to micro-particle penetration were obtained. Measurements of micro-particle penetration depth taken from z-scan image stacks were used to successfully quantify micro-particle distribution within the skin, without invasively disrupting the skin target. This study has shown that MPM has great potential for the non-invasive imaging of particle skin interactive processes that occur with the transdermal delivery of powdered micro-particle vaccines and drugs.
Subjects 090304 Medical Devices
110799 Immunology not elsewhere classified
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Copyright 2002 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

 
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