A model genetic system for testing the in vivo function of peptide toxins

Tedford, Hugo W., Maggio, Francesco, Reenan, Robert A. and King, Glenn F. (2007). A model genetic system for testing the in vivo function of peptide toxins. In: Ronald J. Nachman, Invertebrate Neuropeptides VII: Invertebrate Neuropeptide Conference 2006. 7th Invertebrate Neuropeptide Conference (INC2006), Guanajuato, Mexico, (51-56). 2006. doi:10.1016/j.peptides.2006.08.026


Author Tedford, Hugo W.
Maggio, Francesco
Reenan, Robert A.
King, Glenn F.
Title of paper A model genetic system for testing the in vivo function of peptide toxins
Formatted title
A model genetic system for testing the in vivo function of peptide toxins
Conference name 7th Invertebrate Neuropeptide Conference (INC2006)
Conference location Guanajuato, Mexico
Conference dates 2006
Proceedings title Invertebrate Neuropeptides VII: Invertebrate Neuropeptide Conference 2006   Check publisher's open access policy
Journal name Peptides   Check publisher's open access policy
Place of Publication Philadelphia, PA, United States
Publisher Elsevier
Publication Year 2007
Year available 2006
Sub-type Fully published paper
DOI 10.1016/j.peptides.2006.08.026
Open Access Status Not yet assessed
ISSN 0196-9781
1873-5169
Editor Ronald J. Nachman
Volume 28
Issue 1
Start page 51
End page 56
Total pages 6
Language eng
Abstract/Summary We have developed a model genetic system for analyzing the function of peptide toxins from animal venoms. We engineered and propagated strains of Drosophila melanogaster expressing heat-inducible transgenes encoding either κ-ACTX-Hv1c or ω-ACTX-Hv1a, two insect-specific neurotoxic peptides found in the venom of the Australian funnel-web spider Hadronyche versuta. Heat induction of transgene expression for 20 min was sufficient to kill all transgenic flies, indicating that the ion channels targeted by these toxins are viable insecticide targets. The unusual phenotype of flies induced to express ω-ACTX-Hv1a recapitulates that of a hypomorphic allele of the high-voltage-activated calcium channel Dmca1D, suggesting that this is likely to be the target of ω-ACTX-Hv1a.
Keyword Peptide toxin
Atracotoxin
Drosophila melanogaster
Transgenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 1 December 2006

Document type: Conference Paper
Sub-type: Fully published paper
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 19 May 2009, 02:09:01 EST by Maria Campbell on behalf of Institute for Molecular Bioscience