Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides

He, Lizhong, Robertson, Joseph W. F., Li, Jing, Kärcher, Iris, Schiller, Stefan M., Knoll, Wolfgang and Naumann, Renate (2005) Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides. Langmuir, 21 25: 11666-11672. doi:10.1021/la051771p


Author He, Lizhong
Robertson, Joseph W. F.
Li, Jing
Kärcher, Iris
Schiller, Stefan M.
Knoll, Wolfgang
Naumann, Renate
Title Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides
Journal name Langmuir   Check publisher's open access policy
ISSN 0743-7463
1520-5827
Publication date 2005-12-01
Sub-type Article (original research)
DOI 10.1021/la051771p
Volume 21
Issue 25
Start page 11666
End page 11672
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Subject 100703 Nanobiotechnology
030403 Characterisation of Biological Macromolecules
Abstract Tethered bilayer lipid membranes (tBLMs) are described based on the self-assembly of a monolayer on template stripped gold of an archea analogue thiolipid, 2,3-di-o-phytanyl-sn-glycerol-1-tetraethylene glycol-d,l-α-lipoic acid ester lipid (DPTL), and a newly designed dilution molecule, tetraethylene glycol-d,l-α-lipoic acid ester (TEGL). The tBLM is completed by fusion of liposomes made from a mixture of diphytanoylphosphatidyl choline (DPhyPC), cholesterol, and 1,2-diphytanoyl-sn-glycero-3-phosphate (DPhyPG) in a molar ratio of 6:3:1. Melittin and gramicidin are incorporated into these tBLMs as shown by surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) studies. Ionic conductivity at 0 V vs Ag|AgCl, 3 M KCl, measured by EIS measurements are comparable to the results obtained by other research groups. Admittance plots as a function of potential are discussed on a qualitative basis in terms of the kinetics of ion transport through the channels.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 15 May 2009, 21:36:20 EST by Ms Lynette Adams on behalf of Aust Institute for Bioengineering & Nanotechnology