Using Molecular Tools To Assess Biofilms In Onsite Systems

Flapper, T., McKay, A. and Hall, S. J. (2007). Using Molecular Tools To Assess Biofilms In Onsite Systems. In: Water Science & Technology: Proceeding of the 7th IWA Specialty Conference on Small Water and Wastewater Systems. 7th IWA Specialty Conference on Small Water and Wastewater Systems, Mexico City, Mexico, (87-93). 7-10 March, 2007. doi:10.2166/wst.2007.131


Author Flapper, T.
McKay, A.
Hall, S. J.
Title of paper Using Molecular Tools To Assess Biofilms In Onsite Systems
Conference name 7th IWA Specialty Conference on Small Water and Wastewater Systems
Conference location Mexico City, Mexico
Conference dates 7-10 March, 2007
Convener IWA
Proceedings title Water Science & Technology: Proceeding of the 7th IWA Specialty Conference on Small Water and Wastewater Systems   Check publisher's open access policy
Journal name Water Science and Technology   Check publisher's open access policy
Place of Publication London
Publisher IWA Publishing/Portland Press
Publication Year 2007
Sub-type Fully published paper
DOI 10.2166/wst.2007.131
ISBN 1843395827
ISSN 0273-1223
Volume 55
Issue 7
Start page 87
End page 93
Total pages 7
Language eng
Abstract/Summary Onsite sewage treatment is growing in diversity, and in regulatory control across Australia. This is occurring for both blackwater and greywater treatment, as the drought impact deepens and more of the community are exposed to options for ‘managing’ their own water. Regulators in each State are drafting and implementing Guidelines to cover a range of on-site system scenarios. In addition, more and more decentralised options are being tendered for sewage management in the commercial world. In this project we aim to use novel molecular tools, in combination with traditional physical/chemical/biological methods, to understand onsite treatment in a more detailed manner. The system tested is a new peat based biofilter which can be used for greywater or blackwater application, and can be retrofitted to current sewage systems. This project has been based on the AquaReuse greywater system for demonstration purposes, showing the strength of the information gained from the use of novel tools. The two systems investigated are installed at a caravan park in New South Wales (NSW) and a domestic residence in Tamborine, Queensland (QLD). A 20-week intensive sampling and analysis program was followed. The project monitored standard analytes such as biochemical oxygen demand (BOD5), suspended solids (SS) and thermotolerant coliforms (TC). Additionally, we studied the biological community using fluorescent in situ hybridisation (FISH) on a monthly basis and full-cycle ribosomal RNA analysis (rRNA) for one sample to assess the biological community inhabitants. rRNA analysis at the NSW facility demonstrated a highly diverse biological community, in keeping with its long established operating period. In contrast, FISH analysis at the QLD installation showed a less diverse and younger community. rRNA and FISH identified organisms that are mostly associated with nutrient removing functions.
Subjects 090409 Wastewater Treatment Processes
Keyword Biological
Fish
Greywater
Molecular
Monitoring
rRNA
Q-Index Code E1

 
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