Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha

Nguyen, Vu Tuan, Flanagan, Bernadine M., Gidley, Michael J. and Dykes, Gary A. (2008) Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha. Current Microbiology, 57 5: 449-453. doi:10.1007/s00284-008-9228-3


Author Nguyen, Vu Tuan
Flanagan, Bernadine M.
Gidley, Michael J.
Dykes, Gary A.
Title Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha
Formatted title
Characterization of cellulose production by a Gluconacetobacter xylinus strain from Kombucha
Journal name Current Microbiology   Check publisher's open access policy
ISSN 0343-8651
1432-0991
Publication date 2008-11-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00284-008-9228-3
Open Access Status Not Open Access
Volume 57
Issue 5
Start page 449
End page 453
Total pages 5
Editor Erko Stackebrandt
Place of publication New York, NY, U.S.A.
Publisher Springer
Language eng
Subject C1
860199 Processed Food Products and Beverages (excl. Dairy Products) not elsewhere classified
090899 Food Sciences not elsewhere classified
Abstract The aims of this work were to characterize and improve cellulose production by a Gluconoacetobacter xylinus strain isolated from Kombucha and determine the purity and some structural features of the cellulose from this strain. Cellulose yield in tea medium with both black tea and green tea and in Hestrin and Schramm (HS) medium under both static and agitated cultures was compared. In the tea medium, the highest cellulose yield was obtained with green tea (approximately 0.20 g/L) rather than black tea (approximately 0.14 g/L). Yield in HS was higher (approximately 0.28 g/L) but did not differ between static and agitated incubation. (1)H-NMR and (13)C-NMR spectroscopy indicated that the cellulose is pure (free of acetan) and has high crystallinity, respectively. Cellulose yield was improved by changing the type and level of carbon and nitrogen source in the HS medium. A high yield of approximately 2.64 g/L was obtained with mannitol at 20 g/L and corn steep liquor at 40 g/L in combination. In the tea medium, tea at a level of 3 g/L gave the highest cellulose yield and the addition of 3 g/L of tea to the HS medium increased cellulose yield to 3.34 g/L. In conclusion, the G. xylinus strain from Kombucha had different cellulose-producing characteristics than previous strains isolated from fruit. Cellulose was produced in a pure form and showed high potential applicability. Our studies extensively characterized cellulose production from a G. xylinus strain from Kombucha for the first time, indicating both similarities and differences to strains from different sources.
Formatted abstract
The aims of this work were to characterize and improve cellulose production by a Gluconoacetobacter xylinus strain isolated from Kombucha and determine the purity and some structural features of the cellulose from this strain. Cellulose yield in tea medium with both black tea and green tea and in Hestrin and Schramm (HS) medium under both static and agitated cultures was compared. In the tea medium, the highest cellulose yield was obtained with green tea (∼0.20 g/L) rather than black tea (∼0.14 g/L). Yield in HS was higher (∼0.28 g/L) but did not differ between static and agitated incubation. 1H-NMR and 13C-NMR spectroscopy indicated that the cellulose is pure (free of acetan) and has high crystallinity, respectively. Cellulose yield was improved by changing the type and level of carbon and nitrogen source in the HS medium. A high yield of ∼2.64 g/L was obtained with mannitol at 20 g/L and corn steep liquor at 40 g/L in combination. In the tea medium, tea at a level of 3 g/L gave the highest cellulose yield and the addition of 3 g/L of tea to the HS medium increased cellulose yield to 3.34 g/L. In conclusion, the G. xylinus strain from Kombucha had different cellulose-producing characteristics than previous strains isolated from fruit. Cellulose was produced in a pure form and showed high potential applicability. Our studies extensively characterized cellulose production from a G. xylinus strain from Kombucha for the first time, indicating both similarities and differences to strains from different sources.
© 2008 Springer Science+Business Media, LLC.
Keyword Bacterial cellulose
Acetobacter-xylinum
Agitated culture
Tea
Acetan
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 19 Apr 2009, 23:39:43 EST by Emma Cushworth on behalf of School of Land, Crop and Food Sciences