Encapsulation of DNA and non-viral protein changes the structure of murine polyomavirus virus-like particles

Lipin, D. I., Chuan, Y. P., Lua, L. H. L. and Middelberg, A. P. J. (2008) Encapsulation of DNA and non-viral protein changes the structure of murine polyomavirus virus-like particles. Archives of Virology, 153 11: 2027-2039. doi:10.1007/s00705-008-0220-9


Author Lipin, D. I.
Chuan, Y. P.
Lua, L. H. L.
Middelberg, A. P. J.
Title Encapsulation of DNA and non-viral protein changes the structure of murine polyomavirus virus-like particles
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
1432-8798
Publication date 2008-11-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00705-008-0220-9
Open Access Status
Volume 153
Issue 11
Start page 2027
End page 2039
Total pages 13
Editor M. H. V. Van Regenmortel
Place of publication Wien, Austria
Publisher Springer
Language eng
Subject C1
929999 Health not elsewhere classified
119999 Medical and Health Sciences not elsewhere classified
Abstract Asymmetrical-flow field flow fractionation with multiple-angle light scattering (AFFFF-MALS) was, for the first time, used to characterize the size of murine polyomavirus virus-like particles (MPV VLPs) packaged with either insect cell genomic DNA or non-viral protein. Encapsidation of both genomic DNA and non-viral protein were found to cause a contraction in VLP radii of gyration by approximately 1 nm. Non-viral protein packaged into VLPs consisted of a series of glutathione-S-transferase, His and S tags attached to the N-terminal end of the MPV structural protein VP2 (M (r) = 67108). Transmission electron microscopy analysis of MPV VLPs packaging non-viral protein suggested that VLPs grew in diameter by approximately 5 nm, highlighting the differences between this invasive technique and the relatively non-invasive AFFFF-MALS technique. Encapsulation of non-viral protein into MPV VLPs was found to prevent co-encapsidation of genomic DNA. Further investigation into why this occurred led to the discovery that encapsulation of non-viral protein alters the nuclear localization of MPV VLPs during in vivo assembly. VLPs were relocated away from the ring zone and the nuclear membrane towards the centre of the nucleus amongst the virogenic stroma. The change in nuclear localization away from the site where VLP assembly usually occurs is a likely reason why encapsidation of genomic DNA did not take place.
Keyword Virology
Virology
VIROLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID FF0348465
409976
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemical Engineering Publications
 
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Created: Sat, 18 Apr 2009, 00:42:05 EST by Vicki Thompson on behalf of School of Chemical Engineering