Mesoporous silica templated biolabels with releasable fluorophores for immurroassays

Cai, W. Y., Gentle, I. R., Lu, G. Q., Zhu, J. J. and Yu, A. (2008) Mesoporous silica templated biolabels with releasable fluorophores for immurroassays. ANALYTICAL CHEMISTRY, 80 14: 5401-5406. doi:10.1021/ac800430m

Author Cai, W. Y.
Gentle, I. R.
Lu, G. Q.
Zhu, J. J.
Yu, A.
Title Mesoporous silica templated biolabels with releasable fluorophores for immurroassays
Journal name ANALYTICAL CHEMISTRY   Check publisher's open access policy
ISSN 0003-2700
Publication date 2008-07-15
Year available 2008
Sub-type Article (original research)
DOI 10.1021/ac800430m
Open Access Status
Volume 80
Issue 14
Start page 5401
End page 5406
Total pages 6
Editor Royce W Murray
Place of publication USA
Publisher American Chemical Society
Language eng
Subject C1
860802 Human Diagnostics
100708 Nanomaterials
Abstract A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (similar to 1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was similar to 50 times higher than the conventional dye labelled antibody system.
Keyword Chemistry, Analytical
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes DOI: 10.1021/ac800430m

Document type: Journal Article
Sub-type: Article (original research)
Collection: 2009 Higher Education Research Data Collection
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Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 17 Apr 2009, 22:00:23 EST by Vicki Thompson on behalf of School of Chemical Engineering