Upregulation of brain-derived neurotrophic factor in the sensory pathway by selective motor nerve injury in adult rats

Li, L., Xian, C. J., Zhong, J. H. and Zhou, X. F. (2006) Upregulation of brain-derived neurotrophic factor in the sensory pathway by selective motor nerve injury in adult rats. Neurotoxicity Research, 9 4: 269-283. doi:10.1007/BF03033317


Author Li, L.
Xian, C. J.
Zhong, J. H.
Zhou, X. F.
Title Upregulation of brain-derived neurotrophic factor in the sensory pathway by selective motor nerve injury in adult rats
Journal name Neurotoxicity Research   Check publisher's open access policy
ISSN 1029-8428
1476-3524
Publication date 2006-06-01
Sub-type Article (original research)
DOI 10.1007/BF03033317
Open Access Status DOI
Volume 9
Issue 4
Start page 269
End page 283
Total pages 15
Place of publication Canada
Publisher F. P. Graham Publishing
Language eng
Subject 110904 Neurology and Neuromuscular Diseases
Abstract Selective motor nerve injury by lumbar 5 ventral root transection (L5 VRT) induces neuropathic pain, but the underlying mechanisms remain unknown. Previously, increased expression and secretion of brain-derived neurotrophic factor (BDNF) had been implicated in injury-induced neuropathic pain in the sensory system. In this study, as a step to examine potential roles of BDNF in L5 VRT-induced neuropathic pain, we investigated BDNF gene and protein expression in adult rats with L5 VRT. L5 VRT induced a dramatic upregulation of BDNF mRNA in intact sensory neurons in the ipsilateral L5 dorsal root ganglia (DRG), in non-neuronal cells in the ipsilateral sciatic nerve, and in motoneurons in the ipsilateral spinal cord. L5 VRT also induced de novo synthesis of BDNF mRNA in spinal dorsal horn neurons and in glial cells in the white matter of the ipsilateral spinal cord. Consistent with the mRNA expression pattern, BDNF protein was also mainly upregulated in all populations of sensory neurons in the ipsilateral L5 DRG and in spinal neurons and glia. Quantitative analysis by ELISA showed that the BDNF content in the DRG and sciatic nerve peaked on day 1 and remained elevated 14 days after L5 VRT. These results suggest that increased BDNF expression in intact primary sensory neurons and spinal cord may be an important factor in the induction of neuropathic pain without axotomy of sensory neurons.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Excellence in Research Australia (ERA) - Collection
 
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