Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.

Perneel, Maike, D’hondt, Liesbert, De Maeyer, Katrien, Adiobo, Amayana, Rabaey, Korneel and Hofte, Monica (2008) Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.. Environmental Microbiology, 10 3: 778-788. doi:10.1111/j.1462-2920.2007.01501.x


Author Perneel, Maike
D’hondt, Liesbert
De Maeyer, Katrien
Adiobo, Amayana
Rabaey, Korneel
Hofte, Monica
Title Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.
Formatted title
Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2008-03-01
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2007.01501.x
Volume 10
Issue 3
Start page 778
End page 788
Total pages 11
Editor David A. Stahl
Kenneth N. Timmis
Place of publication Oxford, U.K.
Publisher Blackwell Science
Language eng
Subject C1
969999 Environment not elsewhere classified
060504 Microbial Ecology
0605 Microbiology
Formatted abstract
In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-deficient and a phenazine-deficient mutant of PNA1 were used either separately or jointly in plant experiments. When the mutants were applied separately, no disease-suppressive effect was observed, although both mutants still produced one of the antagonistic compounds (phenazines or rhamnolipids). When the mutants were concurrently introduced in the soil, the biocontrol activity was restored to wild-type levels. Bean seeds developed significantly less pre-emergence damping-off caused by P. splendens when treated with a mixture of purified phenazine-1-carboxamide and rhamnolipids than with any of the chemicals alone. When phenazines and rhamnolipids were combined at concentrations that had no observable effects when the metabolites were applied separately, mycelial growth of P. myriotylum was significantly reduced. In addition, microscopic analysis revealed substantial vacuolization and disintegration of Pythium hyphae after incubation in liquid medium amended with both metabolites. Results of this study indicate that phenazines and biosurfactants are acting synergistically in the control of Pythium spp.
Keyword Phenazines
Pythium spp.
Pseudomonas aeruginosa
Pythium splendens
Phaseolus vulgaris
Pythium myriotylum
Xanthosoma sagittifolium
Biosurfactants
Soil-borne diseases
Biological control
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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