Assessing the use of genomic DNA as a predictor of the maximum absorbance wavelength of avian SWS1 opsin visual pigments

Odeen, Anders, Hart, Nathan S and Hastad, Olle (2009) Assessing the use of genomic DNA as a predictor of the maximum absorbance wavelength of avian SWS1 opsin visual pigments. Journal of Comparative Physiology A: Neuroethology Sensory Neural and Behavioral Physiology, 195 2: 167-173. doi:10.1007/s00359-008-0395-2


Author Odeen, Anders
Hart, Nathan S
Hastad, Olle
Title Assessing the use of genomic DNA as a predictor of the maximum absorbance wavelength of avian SWS1 opsin visual pigments
Journal name Journal of Comparative Physiology A: Neuroethology Sensory Neural and Behavioral Physiology   Check publisher's open access policy
ISSN 0340-7594
Publication date 2009-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00359-008-0395-2
Open Access Status Not yet assessed
Volume 195
Issue 2
Start page 167
End page 173
Total pages 7
Place of publication Berlin, Germany
Publisher Springer
Language eng
Subject C1
060805 Animal Neurobiology
920107 Hearing, Vision, Speech and Their Disorders
0604 Genetics
0608 Zoology
Formatted abstract
Recently, in vitro mutation studies have made it possible to predict the wavelengths of maximum absorbance (λmax) of avian UV/violet sensitive visual pigments (SWS1) from the identity of a few key amino acid residues in the opsin gene. Given that the absorbance spectrum of a cone’s visual pigment and of its pigmented oil droplet can be predicted from just the λmax, it may become possible to predict the entire spectral sensitivity of a bird using genetic samples from live birds or museum specimens. However, whilst this concept is attractive, it must be validated to assess the reliability of the predictions of λmax from opsin amino acid sequences. In this paper, we have obtained partial sequences covering three of the known spectral tuning sites in the SWS1 opsin and predicted λmax of all bird species for which the spectral absorbance has been measured using microspectrophotometry. Our results validate the use of molecular data from genomic DNA to predict the gross differences in λmax between the violet- and ultraviolet-sensitive subtypes of SWS1 opsin. Additionally, we demonstrate that a bird, the bobolink Dolichonyx oryzivorus L., can have more than one SWS1 visual pigment in its retina.
Keyword Colour vision
Microspectrophotometry
MSP
Ultraviolet sensitive
Violet sensitive
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 16 Apr 2009, 21:09:16 EST by Shirley Rey on behalf of Faculty of Science