Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation

Pham, The Hai, Boon, Nico, De Maeyer, Katrien, Höfte, Monica, Rabaey, Korneel and Verstraete, Willy (2008) Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation. Applied Microbiology and Biotechnology, 80 6: 985-993. doi:10.1007/s00253-008-1619-7


Author Pham, The Hai
Boon, Nico
De Maeyer, Katrien
Höfte, Monica
Rabaey, Korneel
Verstraete, Willy
Title Use of Pseudomonas species producing phenazine-based metabolites in the anodes of microbial fuel cells to improve electricity generation
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 2008-10-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00253-008-1619-7
Volume 80
Issue 6
Start page 985
End page 993
Total pages 9
Place of publication Berlin
Publisher Springer
Language eng
Subject C1
9699 Other Environment
090409 Wastewater Treatment Processes
Abstract Abstract The rate of anodic electron transfer is one of the factors limiting the performance of microbial fuel cells (MFCs). It is known that phenazine-based metabolites produced by Pseudomonas species can function as electron shuttles for Pseudomonas themselves and also, in a syntrophic association, for Gram-positive bacteria. In this study, we have investigated whether phenazine-based metabolites and their producers could be used to improve the electricity generation of a MFC operated with a mixed culture. Both anodic supernatants obtained from MFCs operated with a Pseudomonas strain (P-PCA) producing phenazine-1-carboxylic acid (PCA) and those from MFCs operated with a strain (P-PCN) producing phenazine-1-carboxamide (PCN) exerted similarly positive effects on the electricity generation of a mixed culture. Replacing supernatants of MFCs operated with a mixed culture with supernatants of MFCs operated with P-PCN could double the currents generated. Purified PCA and purified PCN had similar effects. If the supernatant of an engineered strain overproducing PCN was used, the effect could be maintained over longer time courses, resulting in a 1.5-fold increase in the production of charge. Bioaugmentation of the mixed culture MFCs using slow release tubes containing P-PCN not only doubled the currents but also maintained the effect over longer periods. The results demonstrated the electron-shuttling effect of phenazine-based compounds produced by Pseudomonas species and their capacity to improve the performance of MFCs operated with mixed cultures.
Keyword Microbial fuel cell
Electrochemically active bacteria
Phenazines
Bacterial interactions
Mixed culture
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Advanced Water Management Centre Publications
 
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Created: Thu, 16 Apr 2009, 21:05:44 EST by Suzanne Read on behalf of Advanced Water Management Centre