Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer

Pham, The Hai, Boon, Nico, Aelterman, Peter, Clauwaert, Peter, De Schamphelaire, Liesje, Vanhaecke, Lynn, deMaeyer, Katrien, Hofte, Monica, Verstaete, Willy and Rabaey, Korneel (2008) Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer. Applied Microbiology and Biotechnology, 77 5: 1119-1129. doi:10.1007/s00253-007-1248-6


Author Pham, The Hai
Boon, Nico
Aelterman, Peter
Clauwaert, Peter
De Schamphelaire, Liesje
Vanhaecke, Lynn
deMaeyer, Katrien
Hofte, Monica
Verstaete, Willy
Rabaey, Korneel
Title Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1007/s00253-007-1248-6
Open Access Status Not yet assessed
Volume 77
Issue 5
Start page 1119
End page 1129
Total pages 11
Place of publication Berlin
Publisher Springer
Language eng
Subject C1
969999 Environment not elsewhere classified
060504 Microbial Ecology
06 Biological Sciences
0605 Microbiology
Abstract Previous studies revealed the abundance of Pseudomonas sp. in the microbial community of a microbial fuel cell (MFC). These bacteria can transfer electrons to the electrode via self-produced phenazine-based mediators. A MFC fed with acetate where several Pseudomonas sp. were present was found to be rich in a Gram-positive bacterium, identified as Brevibacillus sp. PTH1. Remarkably, MFCs operated with only the Brevibacillus strain in their anodes had poor electricity generation. Upon replacement of the anodic aqueous part of Brevibacillus containing MFCs with the cell-free anodic supernatants of MFCs operated with Pseudomonas sp. CMR12a, a strain producing considerable amounts of phenazine-1-carboxamide (PCN) and biosurfactants, the electricity generation was improved significantly. Supernatants of Pseudomonas sp. CMR12a_Reg, a regulatory mutant lacking the ability to produce PCN, had no similar improvement effect. Purified PCN, together with rhamnolipids as biosurfactants (1 mg L−1), could clearly improve electricity generation by Brevibacillus sp. PTH1, as well as enable this bacterium to oxidize acetate with concomitant reduction of ferric iron, supplied as goethite (FeOOH). When added alone, PCN had no observable effects on Brevibacillus’ electron transfer. This work demonstrates that metabolites produced by Pseudomonas sp. enable Gram-positive bacteria to achieve extracellular electron transfer. Possibly, this bacterial interaction is a key process in the anodic electron transfer of a MFC, enabling Brevibacillus sp. PTH1 to achieve its dominance.
Keyword Electrochemically active bacteria
Electron shuttle
Microbial interaction
Phenazines
Biosurfactant
Synergistic effect
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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