PLGA microspheres for the delivery of a novel subunit TB vaccine

Kirby, D. J., Rosenkrands, I., Agger, E. M., Andersen, P., Coombes, A. G. A. and Perrie, Y. (2008) PLGA microspheres for the delivery of a novel subunit TB vaccine. Journal of Drug Targeting, 16 4: 282-293. doi:10.1080/10611860801900462


Author Kirby, D. J.
Rosenkrands, I.
Agger, E. M.
Andersen, P.
Coombes, A. G. A.
Perrie, Y.
Title PLGA microspheres for the delivery of a novel subunit TB vaccine
Journal name Journal of Drug Targeting   Check publisher's open access policy
ISSN 1029-2330
1061-186X
Publication date 2008-01-01
Sub-type Article (original research)
DOI 10.1080/10611860801900462
Open Access Status Not yet assessed
Volume 16
Issue 4
Start page 282
End page 293
Total pages 11
Editor Akhtar, S.
Place of publication United Kingdom
Publisher Informa Healthcare
Language eng
Subject C1
111504 Pharmaceutical Sciences
860801 Human Biological Preventatives (e.g. Vaccines)
Abstract Biodegradable poly(dl-lactide-co-glycolide) microspheres were prepared using a modified double emulsion solvent evaporation method for the delivery of the subunit tuberculosis vaccine (Ag85B-ESAT-6), a fusion protein of the immunodominant antigens 6-kDa early secretory antigenic target (ESAT-6) and antigen 85B (Ag85B). Addition of the cationic lipid dimethyl dioctadecylammonium bromide (DDA) and the immunostimulatory trehalose 6,6'-dibehenate (TDB), either separately or in combination, was investigated for the effect on particle size and distribution, antigen entrapment efficiency, in vitro release profiles and in vivo performance. Optimised formulation parameters yielded microspheres within the desired sub-10 μm range (1.50 ± 0.13 μm), whilst exhibiting a high antigen entrapment efficiency (95 ± 1.2%) and prolonged release profiles. Although the microsphere formulations induced a cell-mediated immune response and raised specific antibodies after immunisation, this was inferior to the levels achieved with liposomes composed of the same adjuvants (DDA-TDB), demonstrating that liposomes are more effective vaccine delivery systems compared with microspheres.
Keyword Microspheres
PLGA
adjuvants
subunit vaccines
tuberculosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 35 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 16 Apr 2009, 19:49:52 EST by Elizabeth Pyke on behalf of School of Pharmacy