Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass

Davies, J. M., Bright, M. L., Rolland, J. M. and O'Hehir, R. E. (2005) Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass. Allergy, 60 2: 251-255. doi:10.1111/j.1398-9995.2005.00663.x


Author Davies, J. M.
Bright, M. L.
Rolland, J. M.
O'Hehir, R. E.
Title Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass
Journal name Allergy   Check publisher's open access policy
ISSN 0105-4538
1398-9995
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1111/j.1398-9995.2005.00663.x
Open Access Status Not yet assessed
Volume 60
Issue 2
Start page 251
End page 255
Total pages 5
Place of publication Copenhagen, Denmark
Publisher Blackwell Munksgaard
Language eng
Subject 11 Medical and Health Sciences
1107 Immunology
Formatted abstract
Background:
Perennial Ryegrass is a major cause of rhinitis in spring and early summer. Bahia grass, Paspalum notatum, flowers late into summer and could account for allergic rhinitis at this time. We determined the frequency of serum immunoglobulin (Ig)E reactivity with Bahia grass in Ryegrass pollen allergic patients and investigated IgE cross-reactivity between Bahia and Ryegrass.

Methods:
Serum from 33 Ryegrass pollen allergic patients and 12 nonatopic donors were tested for IgE reactivity with Bahia and Ryegrass pollen extracts (PE) by enzyme-linked immunosorbent assay (ELISA), western blotting and inhibition ELISA. Allergen-specific antibodies from a pool of sera from allergic donors were affinity purified and tested for IgE cross-reactivity.

Results:
Seventy-eight per cent of the sera had IgE reactivity with Bahia grass, but more weakly than with Ryegrass. Antibodies eluted from the major Ryegrass pollen allergens, Lol p 1 and Lol p 5, showed IgE reactivity with allergens of Ryegrass and Canary but not Bahia or Bermuda grasses. Timothy, Canary and Ryegrass inhibited IgE reactivity with Ryegrass and Bahia grass, whereas Bahia, Johnson and Bermuda grass did not inhibit IgE reactivity with Ryegrass.

Conclusions:
The majority of Ryegrass allergic patients also showed serum IgE reactivity with Bahia grass PE. However, Bahia grass and Ryegrass had only limited IgE cross-reactivity indicating that Bahia grass should be considered in diagnosis and treatment of patients with hay fever late in the grass pollen season.
Keyword Bahia grass
Immunoglobulin E cross-reactivity
Ryegrass
Seasonal rhinitis
Serum immunoglobulin E
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 16 Apr 2009, 19:30:22 EST by Ms Sarada Rao on behalf of Faculty Of Health Sciences