Toward a full characterization of native starch: Separation and detection by size-exclusion chromatography

Hoang, Ngoc-Ly, Landolfi, Antonin., Kravchuk, Anastasiya., Girard, Etienne, Peate, Jonathon., Hernandez, Javier. M., Gaborieau, Marianne., Kravchuk, Olena, Gilbert, Robert. G., Guillaneuf, Yohann. and Castignolles, Patrice . (2008) Toward a full characterization of native starch: Separation and detection by size-exclusion chromatography. Journal of Chromatography A, 1205 1-2: 60-70. doi:10.1016/j.chroma.2008.07.090


Author Hoang, Ngoc-Ly
Landolfi, Antonin.
Kravchuk, Anastasiya.
Girard, Etienne
Peate, Jonathon.
Hernandez, Javier. M.
Gaborieau, Marianne.
Kravchuk, Olena
Gilbert, Robert. G.
Guillaneuf, Yohann.
Castignolles, Patrice .
Title Toward a full characterization of native starch: Separation and detection by size-exclusion chromatography
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 2008-09-26
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.chroma.2008.07.090
Open Access Status
Volume 1205
Issue 1-2
Start page 60
End page 70
Total pages 11
Editor Poole, C.F
Dorsey, J.G.
Geise, R.W.
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
860199 Processed Food Products and Beverages (excl. Dairy Products) not elsewhere classified
030399 Macromolecular and Materials Chemistry not elsewhere classified
Abstract The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
Keyword Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, ANALYTICAL
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP 665360
Institutional Status UQ

 
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Created: Thu, 16 Apr 2009, 03:03:09 EST by Emma Cushworth on behalf of School of Land, Crop and Food Sciences