Plant cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae

Barbeta, Barbara L., Marshall, Alan T., Gillon, Amanda D., Craik, David J. and Anderson, Marilyn A. (2008) Plant cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae. Proceedings of the National Acadamy of Sciences of the United State of America, 105 4: 1221-1225. doi:10.1073/pnas.0710338104


Author Barbeta, Barbara L.
Marshall, Alan T.
Gillon, Amanda D.
Craik, David J.
Anderson, Marilyn A.
Title Plant cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae
Journal name Proceedings of the National Acadamy of Sciences of the United State of America   Check publisher's open access policy
ISSN 0027-8424
Publication date 2008-01-29
Year available 2008
Sub-type Article (original research)
DOI 10.1073/pnas.0710338104
Open Access Status Not Open Access
Volume 105
Issue 4
Start page 1221
End page 1225
Total pages 4
Place of publication Washington, DC, United States
Publisher The Academy
Language eng
Subject C1
030406 Proteins and Peptides
860703 Crop Protection Chemicals
Abstract Several members of the Rubiaceae and Violaceae plant families produce a series of cyclotides or macrocyclic peptides of 28–37 aa with an embedded cystine knot. The cyclic peptide backbone together with the knotted and strongly braced structure confers exceptional chemical and biological stability that has attracted attention for potential pharmaceutical applications. Cyclotides display a diverse range of biological activities, such as uterotonic action, anti-HIV activity, and neurotensin antagonism. In plants, their primary role is probably protection from insect attack. Ingestion of the cyclotide kalata B1 severely retards the growth of larvae from the Lepidopteran species Helicoverpa armigera. We examined the gut of these larvae after consumption of kalata B1 by light, scanning, and transmission electron microscopy. We established that kalata B1 induces disruption of the microvilli, blebbing, swelling, and ultimately rupture of the cells of the gut epithelium. The histology of this response is similar to the response of H. armigera larvae to the Bacillus thuringiensis delta-endotoxin, which is widely used to control these insect pests of crops such as cotton.
Keyword Circular peptides
Insecticidal
Kalata B1
Microscopy
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 16 Apr 2009, 02:23:41 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience